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New Forum Archives (2009-)
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Protein-and-Proteomics
31.
BCA microplate assay with 595 nm filter? -
(reply: 1)
32.
Cannot figure this dot blot thing out -
(reply: 2)
33.
Stability of a typical protein at 4oC? -
(reply: 1)
34.
Uses of ion exchange chromatography? -
(reply: 1)
35.
Friendly antibody buffer to prevent precipitation? -
(reply: 3)
36.
Use of fluorescent stain on IEF gels -
(reply: 1)
37.
Use of speedvac to dry in-gel peptide digest before sending for LCMS -
(reply: 2)
38.
doublets on 2D gel -
(reply: 4)
39.
RNA later and 2D SDS PAGE -
(reply: 3)
40.
Peptide quantification -
(reply: 4)
41.
Affinity purification glycine elution chemistry -
(reply: 3)
42.
Zeba buffer exchange low protein yield -
(reply: 6)
43.
iTRAQ or ELISA? -
(reply: 1)
44.
2D Quant kit -
(reply: 2)
45.
2D PAGE and Western blotting -
(reply: 1)
46.
How to detect specific-protein producing cells? -
(reply: 8)
47.
continual problems with 2d gel electrophoresis -
(reply: 3)
48.
Gradient centrifugation -
(reply: 1)
49.
Liquid nitrogen protein extraction -
(reply: 3)
50.
protein extraction from lettuce -
(reply: 1)
51.
Protein Clevage -
(reply: 1)
52.
yeast two hybrid 3-AT -
(reply: 1)
53.
Protein expression -
(reply: 1)
54.
Protein denaturation -
(reply: 5)
55.
Human cell membrane protein Western blot! -
(reply: 2)
56.
protein staining with EtBr!! -
(reply: 4)
57.
large scale protein purification -
(reply: 6)
58.
Insoluble protein after ammonium sulfate precipitation -
(reply: 1)
59.
General questions for enzyme assay -
(reply: 2)
60.
Question regarding protein conservation among similar genes -
(reply: 2)
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