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811. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
812. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
813. EMSA probes - method to make longer probes (reply: 3)
814. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
815. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
816. GST pull down - (reply: 1)
817. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
818. Determining how much enzyme to use based on activity units - (reply: 3)
819. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
820. yield vs concentration of protein - (reply: 3)
821. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
822. Sonication mysteries - (reply: 4)
823. cys rich protein expression - (reply: 1)
824. immunoprecipitation troubleshooting - (reply: 1)
825. Immunoprecipitation with two antibodies - (reply: 10)
826. RIPA Buffer precipitation trouble - (reply: 5)
827. develop western by ECL plus - (reply: 1)
828. Protein extraction from tissue - (reply: 1)
829. problem of membrane protein FPLC - (reply: 1)
830. spot removal from 2D gel and proteomics - help! (reply: 1)
831. Co-IP problem - (reply: 3)
832. I am confused, please help. - (reply: 1)
833. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
834. EMSA using biotin labeled probes and buffers - (reply: 5)
835. Foam in the BSA solution - (reply: 3)
836. Inclusion Body purification - (reply: 2)
837. How to design an antibody - (reply: 1)
838. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
839. Protein isolation from skin - (reply: 2)
840. How to concentrate my protein? - (reply: 1)
812. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
813. EMSA probes - method to make longer probes (reply: 3)
814. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
815. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
816. GST pull down - (reply: 1)
817. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
818. Determining how much enzyme to use based on activity units - (reply: 3)
819. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
820. yield vs concentration of protein - (reply: 3)
821. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
822. Sonication mysteries - (reply: 4)
823. cys rich protein expression - (reply: 1)
824. immunoprecipitation troubleshooting - (reply: 1)
825. Immunoprecipitation with two antibodies - (reply: 10)
826. RIPA Buffer precipitation trouble - (reply: 5)
827. develop western by ECL plus - (reply: 1)
828. Protein extraction from tissue - (reply: 1)
829. problem of membrane protein FPLC - (reply: 1)
830. spot removal from 2D gel and proteomics - help! (reply: 1)
831. Co-IP problem - (reply: 3)
832. I am confused, please help. - (reply: 1)
833. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
834. EMSA using biotin labeled probes and buffers - (reply: 5)
835. Foam in the BSA solution - (reply: 3)
836. Inclusion Body purification - (reply: 2)
837. How to design an antibody - (reply: 1)
838. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
839. Protein isolation from skin - (reply: 2)
840. How to concentrate my protein? - (reply: 1)