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Protein-and-Proteomics
691.
Quantitative Western Imaging -
(reply: 3)
692.
What causes this offset band on RIPA gel? -
(reply: 4)
693.
What portion of protein can I get under this protocol? -
(reply: 3)
694.
Squished lanes in SDS Page -
(reply: 4)
695.
Protin helicase (urgent) - helicase activity after long term storage
(reply: 3)
696.
BN-PAGE -
(reply: 2)
697.
How can I get protein in soluble fration -
(reply: 2)
698.
How long does it takes to make a crystallography of a protein? -
(reply: 1)
699.
how to detect a knowen protein's receptor? -
(reply: 1)
700.
protein purification - removing salts, detergents, etc from a protein sample
(reply: 6)
701.
western blot to a metal mesh -
(reply: 3)
702.
Protein quantification -
(reply: 2)
703.
Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods
(reply: 1)
704.
Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps
(reply: 4)
705.
Custom Primary Antibody -
(reply: 3)
706.
substrate for kinase - finding the predicted substrate for a kinase
(reply: 6)
707.
when should I add IPTG? -
(reply: 2)
708.
No protein spots in 2D gel - Silver staining! -
(reply: 17)
709.
appropriate time for induction (adding IPTG) -
(reply: 1)
710.
Help!! BCA kit v.s Bio-Rad kit for protein assay -
(reply: 1)
711.
protein crystallography services question -
(reply: 1)
712.
Probelm with gel filtration -
(reply: 4)
713.
c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection
(reply: 2)
714.
What temperature should DTT be stored (4oC or -20oC)? -
(reply: 4)
715.
best and simple method to purify protein -
(reply: 38)
716.
Pipet trypsin tips -
(reply: 3)
717.
Are proteases a problem in protein quantification assays? -
(reply: 3)
718.
serum housekeeping proteins for total protein normalization -
(reply: 1)
719.
inclusion body purification kit - GST tagged insoluble proteins
(reply: 2)
720.
Co-IP on nuclear fraction -
(reply: 2)
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