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571. Single amino acid substitutions away from the active site causing disease. - (reply: 4)
572. how to express protein in soluble form - (reply: 1)
573. IP no target protein in elution - (reply: 1)
574. Protein stability - (reply: 4)
575. Relation between microns and kD - (reply: 3)
576. ATP assay - malachite green - (reply: 3)
577. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
578. Protein isolation in zebrafish - (reply: 1)
579. Acetone washes for TCA precipitation protocoles - (reply: 3)
580. Biotinylation of protein in tris buffer - (reply: 2)
581. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
582. Protein kinase protocols - (reply: 2)
583. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
584. Purification of His tagged protein - (reply: 2)
585. Protease and proteasome inhibitors - (reply: 2)
586. BCA diluent and standard curve - (reply: 1)
587. protein induction problem - (reply: 4)
588. Growing insect cells with other cell lines - (reply: 2)
589. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
590. Protein expression declines after overnight incubation - (reply: 4)
591. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
592. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
593. Fusion protein linker - (reply: 2)
594. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
595. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
596. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
597. Protein streaking on Blue Native-PAGE - (reply: 9)
598. basic questions about protein assays and cuvettes - (reply: 4)
599. How to attain pure chains of one protein complex - (reply: 1)
600. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
572. how to express protein in soluble form - (reply: 1)
573. IP no target protein in elution - (reply: 1)
574. Protein stability - (reply: 4)
575. Relation between microns and kD - (reply: 3)
576. ATP assay - malachite green - (reply: 3)
577. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
578. Protein isolation in zebrafish - (reply: 1)
579. Acetone washes for TCA precipitation protocoles - (reply: 3)
580. Biotinylation of protein in tris buffer - (reply: 2)
581. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
582. Protein kinase protocols - (reply: 2)
583. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
584. Purification of His tagged protein - (reply: 2)
585. Protease and proteasome inhibitors - (reply: 2)
586. BCA diluent and standard curve - (reply: 1)
587. protein induction problem - (reply: 4)
588. Growing insect cells with other cell lines - (reply: 2)
589. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
590. Protein expression declines after overnight incubation - (reply: 4)
591. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
592. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
593. Fusion protein linker - (reply: 2)
594. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
595. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
596. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
597. Protein streaking on Blue Native-PAGE - (reply: 9)
598. basic questions about protein assays and cuvettes - (reply: 4)
599. How to attain pure chains of one protein complex - (reply: 1)
600. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)