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Protein-and-Proteomics
301.
sample preparation from whole cell lysates for Edman degradation -
(reply: 2)
302.
Role of Isoleucine in protein structure. -
(reply: 1)
303.
co-IP after ubiquitin cleavage -
(reply: 5)
304.
Heterologous gene expression in yeast - a bioinformatician needs your help cloni -
(reply: 1)
305.
Peptide Conc @215 and 225nm? -
(reply: 1)
306.
Insoluble material floats over my SDS lysate of human cells after centrifugation -
(reply: 7)
307.
Detection method of insulin level from blood serum -
(reply: 2)
308.
Relationship between total protein and enzyme activity -
(reply: 3)
309.
about cross link the antibody to ProteinG -
(reply: 1)
310.
EMSA supershift problem -
(reply: 1)
311.
Co-IP: Prey Protein Present in Neg and Pos Eluates -
(reply: 5)
312.
covalent-bound HIS complex can not be pull down: help needed -
(reply: 2)
313.
Protein extraction from mouse tissue? -
(reply: 1)
314.
Including "M" and "STOP" codons in my insert -
(reply: 2)
315.
Coomassie stain forzen and thawed gel? -
(reply: 1)
316.
IEF gel detaching during run -
(reply: 7)
317.
Overexpressed protein shows a band higher than expected?! -
(reply: 2)
318.
Bradford Protein Assay - Problem in BSA Standard Curve -
(reply: 2)
319.
HIS elution: can imidazole be added after SDS-sample buffer? -
(reply: 1)
320.
What is Pellet that forms AFTER quick spin of boiled lysates? -
(reply: 1)
321.
ethanol precipitation of protein -
(reply: 1)
322.
How to thoroughly solubilize proteins from tissue sample, for quantitation -
(reply: 1)
323.
Protein Concentration from Bacterial Lysates: Help! -
(reply: 2)
324.
Is DTT reduction and alkylation critical before concentrating proteins? -
(reply: 1)
325.
Tricine SDS-PAGE -
(reply: 3)
326.
Pichia growth in MGY/MGYH media -
(reply: 1)
327.
protien purification using flag affinity -
(reply: 4)
328.
Constitutive p42/44 MAPK phosphorylation -
(reply: 2)
329.
His Tag Cleavage (protein precipitates) -
(reply: 3)
330.
Lysis buffer for Co-IP -
(reply: 2)
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