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631. GST pulldown: elude or not? - (reply: 1)
632. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
633. secreted protein quantification - (reply: 2)
634. Am. Sulp Precipitation - (reply: 2)
635. EMSA - Probe Annealing problems (reply: 1)
636. Mass spectrometry for dummies? - (reply: 3)
637. Major Protein Loss! Why? - (reply: 2)
638. Filming membrane (Western Blot & ECL) - (reply: 1)
639. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
640. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
641. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
642. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
643. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
644. protein purification - (reply: 5)
645. western blot is not consistent with immunofluorescence - (reply: 2)
646. determination of the Stoichiometry - (reply: 1)
647. cytoplasmic and nuclear extraction for BCA - (reply: 6)
648. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
649. protein prep - about prptein preperation in diagnosis (reply: 1)
650. blocking for 48hours - (reply: 1)
651. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
652. SDS PAGE - Bubble inside the Gel (reply: 5)
653. loss of protein during concentration - (reply: 6)
654. protease inhibitors and bradford - (reply: 2)
655. Cell lysates - Protein precipitation? - (reply: 3)
656. Which lysis buffer should I use? - (reply: 5)
657. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
658. cell lysate for pulldown - (reply: 3)
659. two hybrid system - opinion (reply: 1)
660. How many kDa add a post-translation modification? - (reply: 2)
632. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
633. secreted protein quantification - (reply: 2)
634. Am. Sulp Precipitation - (reply: 2)
635. EMSA - Probe Annealing problems (reply: 1)
636. Mass spectrometry for dummies? - (reply: 3)
637. Major Protein Loss! Why? - (reply: 2)
638. Filming membrane (Western Blot & ECL) - (reply: 1)
639. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
640. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
641. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
642. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
643. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
644. protein purification - (reply: 5)
645. western blot is not consistent with immunofluorescence - (reply: 2)
646. determination of the Stoichiometry - (reply: 1)
647. cytoplasmic and nuclear extraction for BCA - (reply: 6)
648. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
649. protein prep - about prptein preperation in diagnosis (reply: 1)
650. blocking for 48hours - (reply: 1)
651. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
652. SDS PAGE - Bubble inside the Gel (reply: 5)
653. loss of protein during concentration - (reply: 6)
654. protease inhibitors and bradford - (reply: 2)
655. Cell lysates - Protein precipitation? - (reply: 3)
656. Which lysis buffer should I use? - (reply: 5)
657. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
658. cell lysate for pulldown - (reply: 3)
659. two hybrid system - opinion (reply: 1)
660. How many kDa add a post-translation modification? - (reply: 2)