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1. GTPase assay - (reply: 2)
2. eliminating albumin from samples for western blotting ?. - (reply: 1)
3. Liquid nitrogen protein extraction ?. - (reply: 2)
4. Standard HCL protein hydrolysis technique without vacuum - (reply: 1)
5. how to enhance the detection limit of home-made Bradford reagent? - (reply: 2)
6. Problem with protein induction - (reply: 2)
7. Percentage of Outer Membrane Protein - (reply: 2)
8. Storage period of protein - (reply: 1)
9. Reduction of Foam formation - (reply: 1)
10. Outer membrane protein extraction - (reply: 1)
11. HEPES BUFFER and protein degradation - (reply: 1)
12. coIP troubleshooting - (reply: 1)
13. Magnetic beads vs Sepharose beads - (reply: 1)
14. Whole brain homogenziation for coIP - (reply: 1)
15. Immunoprecipitation protocol - (reply: 2)
16. coIP followed by a Western Blot - (reply: 4)
17. Reverse coIP - (reply: 5)
18. N-terminus tagging and signalP prediction - (reply: 2)
19. how to predict transmembrane protein structure - (reply: 7)
20. slimy cell paste of recombinant e.coli - (reply: 3)
21. n=3 for pSILAC - (reply: 1)
22. Is there any significant difference between endogenous/ native promoter and cons - (reply: 1)
23. kDa - (reply: 1)
24. Salting out specific protein - (reply: 1)
25. Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
26. Bradford assay which is the blank? - (reply: 7)
27. protein sequence mutagenesis - (reply: 2)
28. Mass Spectrometry help needed - (reply: 1)
29. Protein quantification using HPLC/fluorescence - (reply: 3)
30. Effect on protein of low pH? - (reply: 1)
2. eliminating albumin from samples for western blotting ?. - (reply: 1)
3. Liquid nitrogen protein extraction ?. - (reply: 2)
4. Standard HCL protein hydrolysis technique without vacuum - (reply: 1)
5. how to enhance the detection limit of home-made Bradford reagent? - (reply: 2)
6. Problem with protein induction - (reply: 2)
7. Percentage of Outer Membrane Protein - (reply: 2)
8. Storage period of protein - (reply: 1)
9. Reduction of Foam formation - (reply: 1)
10. Outer membrane protein extraction - (reply: 1)
11. HEPES BUFFER and protein degradation - (reply: 1)
12. coIP troubleshooting - (reply: 1)
13. Magnetic beads vs Sepharose beads - (reply: 1)
14. Whole brain homogenziation for coIP - (reply: 1)
15. Immunoprecipitation protocol - (reply: 2)
16. coIP followed by a Western Blot - (reply: 4)
17. Reverse coIP - (reply: 5)
18. N-terminus tagging and signalP prediction - (reply: 2)
19. how to predict transmembrane protein structure - (reply: 7)
20. slimy cell paste of recombinant e.coli - (reply: 3)
21. n=3 for pSILAC - (reply: 1)
22. Is there any significant difference between endogenous/ native promoter and cons - (reply: 1)
23. kDa - (reply: 1)
24. Salting out specific protein - (reply: 1)
25. Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
26. Bradford assay which is the blank? - (reply: 7)
27. protein sequence mutagenesis - (reply: 2)
28. Mass Spectrometry help needed - (reply: 1)
29. Protein quantification using HPLC/fluorescence - (reply: 3)
30. Effect on protein of low pH? - (reply: 1)