BCA microplate assay with 595 nm filter? - (Apr/06/2016 )
I want to do a BCA protein microplate assay (pierce) but our plate reader only has a 595 nm filter. The manufacturer of the BCA kit advises to use a 562 nm filter, or at most a 590 nm filter. Has anyone successfully used a 595 nm filter for this assay?
-Michal Levy-
I used two different BCA kit before, CreativeBiomart and Beyotime. Here are their products descriptions:
http://www.creativebiomart.net/BCA-Protein-Quantitation-Kit-462389.htm
http://download.bioon.com.cn/view/upload/201304/27110325_7082.pdf
In the PDF, OD562nm is best choice. But 540-595nm are also suitable for their products. I'm not sure whether 595nm also suitable for your kit.
-Andrea Fortina-