|
|
QPCR Related Discussions
-
qPCR problems with chIP samples - (reply: 2)
-
qPCR delta delta CT calculation - (reply: 1)
-
qRT-PCR for pre- and pri-miRNAs - (reply: 2)
-
DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
-
normalizing between qPCR runs - (reply: 2)
-
mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
-
qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
-
qPCR troubles - (reply: 5)
-
qPCR 96 well plate - (reply: 2)
-
What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
-
qRT-PCR primer design - (reply: 1)
-
QPCR data anlaysis - (reply: 4)
-
Problems with second use of diluted cDNA in qPCR - (reply: 4)
-
qRT-PCR - data analysis - methods that DO NOT use a calibrator ! (reply: 3)
-
First strand cDNA synthesis for qRT-PCR - (reply: 1)
-
qRT-PCR control - (reply: 1)
-
Difference between semi-q and qPCR - (reply: 2)
-
Multichannel Pipette for qPCR plates - Effective manual pipetting of qPCR plates (reply: 1)
-
Stratagene MX4000 qPCR plates to give away - UK only & preferably pick up in Cambridge (reply: 5)
-
Magnetic beads in a QPCR rxn - (reply: 1)
-
qPCR internal control - (reply: 3)
-
unsolved qPCR problem! - (reply: 1)
-
how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
-
qRT-PCR: how to average multiple runs? - (reply: 1)
-
QPCR - (reply: 9)
-
QPCR - (reply: 2)
-
qPCR - (reply: 1)
-
New to qPCR - (reply: 2)
-
Low Ct value in ChIP qPCR - (reply: 4)
-
real-time PCR problem - (reply: 1)
-
Real-time PCR for mRNA stability - (reply: 2)
-
qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
-
qRT-PCR reproducibility of triplicates? - (reply: 6)
-
miRNA real-time using Sybr green - (reply: 9)
-
How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
-
qPCR standard curve problem - (reply: 1)
-
Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
-
how to optimize primer and cDNA concentration for real-time - (reply: 2)
-
Problem with qPCR of ChIP input - (reply: 12)
-
Using real-time to differentiate between SNPs - (reply: 5)
-
Bad primer efficiency during multiplex qPCR - (reply: 1)
-
What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
-
qRT-PCR first timer, controls? - (reply: 9)
-
ChIP and qPCR - ChIP and qPCR (reply: 4)
-
Ki67 IHC vs Real-time PCR - studying proliferation (reply: 1)
-
Fluorescence level in real-time - (reply: 2)
-
What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
-
miRNA array data and QPCR data correlation - (reply: 6)
-
need help on microRNA real-time PCR - (reply: 6)
-
standard curve method for relative qPCR quantitation - (reply: 2)
-
RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
-
Primer concentration is quantitative Real-time PCR - (reply: 1)
-
choosing real-time PCR machine - (reply: 13)
-
Statistics help with real-time relative quantification (RQ) values - (reply: 2)
-
Real-Time PCR and Housekeeping gene - (reply: 9)
-
ChIP and qRT-PCR - (reply: 1)
-
Controls for real-time PCR... again - (reply: 7)
-
ChIP qPCR data analysis? - (reply: 9)
-
help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
-
absolute qPCR problem - (reply: 1)
-
Simple QPCR question? - (reply: 1)
-
Detection of LNA miRNA knock-down by qPCR? - (reply: 5)
-
Taqman qpcr sensitivity - (reply: 1)
-
Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
-
RT-PCR Vs qRT-PCR help please! - (reply: 4)
-
SYBR green concentration for qPCR - (reply: 2)
-
PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
-
qrt-pcr on bacterial transcripts - (reply: 1)
-
Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
-
qPCR on low to non-existant template - (reply: 4)
-
elp with qPCR standard curve - (reply: 5)
-
HELP with qPCR results - (reply: 3)
-
QPCR primer Pairs - (reply: 1)
-
qPCR Exp. Design - Reruning Exp. Control Samples - (reply: 3)
-
Statistics: real-time RT-PCR - (reply: 4)
-
QPCR versus regular PCR for Chip - (reply: 2)
-
what is meaning of true enrichment for chip qPCR? - (reply: 5)
-
MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
-
Help for Real-time PCR equipment choice - (reply: 4)
-
urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
-
qRT-PCR help! - (reply: 2)
-
qRT-PCR and Northern blot - Is miRNA Northern blot necessary? (reply: 2)
-
gene-specific Primer for qRT-PCR - (reply: 2)
-
No signal in Real-time but in normal PCR?! - (reply: 2)
-
need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
-
Real-time PCR low efficiency - (reply: 1)
-
qPCR databases - (reply: 1)
-
qPCR and plasmid positive controls - Why use plasmid positive controls? Are they the best? If so, are there (reply: 1)
-
QPcr for alternatively spliced products - (reply: 4)
-
What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
-
qPCR primer efficiancy - (reply: 2)
-
ChIP and qPCR calculation ? - (reply: 8)
-
Touchdown conditions for qPCR - (reply: 1)
-
QPCR Quantitation of copy number - (reply: 1)
-
ELISA in a real-time PCR plate - possible? (reply: 3)
-
genomic DNA for qPCR quantification - target sequence? - (reply: 1)
-
looking for a website for a newbie to learn qPCR - (reply: 3)
-
qPCR for microRNAs - minimal primer length? (reply: 6)
-
Real-Time MSP - (reply: 1)
-
preference for region of primer design for qRT-PCR - (reply: 1)
-
qPCR housekeepers - primers or probes? - (reply: 1)
-
QPCR Oligo Database of Pathogens - (reply: 1)
-
what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
-
Free sample from Takara. - All kind of polymerase for PCR and real-time PCR. (reply: 7)
-
Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
-
real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
-
Real-time PCR optimization - (reply: 8)
-
Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
-
Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
-
Real-time PCR....Making sense of it all? - (reply: 3)
-
Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
-
QPCR with MSP - (reply: 3)
-
Real-time PCR to analyze DNA methylation - (reply: 3)
-
qPCR using random primers - (reply: 4)
-
Verification of real-time amplification specificity - (reply: 1)
-
Problems with RNA extraction or QPCR for AI - (reply: 2)
-
miRNA QPCR advice - taqman (reply: 3)
-
No-RT control in qRT-PCR - (reply: 5)
-
Longest Amplicon Using Real-Time PCR? - (reply: 1)
-
cDNA for real-time PCR - (reply: 3)
-
DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
-
PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
-
normalization for real-time PCR - (reply: 4)
-
One-step versus two-step qRT-PCR - (reply: 5)
-
primer for real-time and reverse transcriptase PCR - (reply: 1)
-
Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
-
qPCR vs RNA protection assay - (reply: 1)
-
Plasmid for qPCR standard curve - (reply: 3)
-
Can you use Picogreen in a QPCR instrument? - (reply: 1)
-
Real-Time PCR Question - (reply: 1)
-
Target Amplicon Length for real-time PCR - (reply: 4)
-
Real-Time PCR/Melting Curve Question - (reply: 3)
-
can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
-
Real-Time PCR Amplicon Question - (reply: 2)
-
need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
-
Diluting your cDNA for qRT-PCR? - (reply: 5)
-
RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
-
Help! Opticon real-time machines thinks I only want to do 1 cycle! - (reply: 3)
-
What about the BIO-RAD's real-time machine? - (reply: 2)
-
How can I prepare standard DNA for QPCR - (reply: 3)
-
Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
-
qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
-
Standard Curve Issues with ABI 7500 qPCR - (reply: 4)
-
Pre-Preparation of qPCR plates - Pros and Cons (reply: 4)
-
ChIP qPCR analysis - (reply: 2)
-
housekeeping gene in real-time PCR - (reply: 2)
-
Trouble with real-time PCR standard curve - (reply: 4)
-
2 melting peaks in real-time PCR - (reply: 1)
-
qPCR with Corbett Rotor Gene 6000 - (reply: 3)
-
Relative Quantification in real-time RT-PCR - (reply: 5)
-
mirVana qRT-PCR with the Roche 1.5 LightCycler - (reply: 1)
-
Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
-
NTC's in RT or just qPCR? - (reply: 3)
-
Can't repeat a perfect miRNA real-time standard curve - (reply: 2)
-
qPCR Efficiencies over 100% - Looking for references (reply: 1)
-
real-time PCR for quantitation of MSP - (reply: 3)
-
qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
-
Small amounts of RNA for qRT-PCR - (reply: 7)
-
qPCR Standard control - plasmid quantify (reply: 3)
-
Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
-
how long should qPCR product be? - 150~250bp? (reply: 2)
-
Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
-
Strange thing about RNA concentration and real-time melting curve - (reply: 4)
-
random primers for qPCR - (reply: 3)
-
QPCR, where to start? - optimizing primer and template (reply: 5)
-
qPCR standards - (reply: 1)
-
Taqman QPCR help - amplification of FAM right from the 0 cycle (reply: 5)
-
QPCR Copy number - (reply: 2)
-
RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
-
qPCR detection for single nucleotide substitution - (reply: 5)
-
Best product size for real-time RT-PCR - (reply: 4)
-
Which housekeeping genes for qRT-PCR control? - (reply: 17)
-
Replication of qRT-PCR experiments - (reply: 5)
-
generation of gDNA Std curves in qPCR - ABI 7300 qPCR (reply: 1)
-
Minimum amount of RNA for real-time PCR - (reply: 4)
-
is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
-
genomic dna in real-time pcr - (reply: 3)
-
What's the function of UNG in qPCR? - (reply: 6)
-
What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
-
ChIP quantification (qPCR) - (reply: 2)
-
Not enough cDNA for qRT-PCR - how to increase cDNA yield or save cDNA? - (reply: 12)
-
Self-made qRT-PCR enzyme-/mastermix? - (reply: 8)
-
Home-made Sybr green mix for real-time PCR - (reply: 1)
-
Should I dilute cDNA for real-time PCR? - (reply: 3)
-
Real-time PCR problems - RT_PCR (reply: 7)
-
strange contamination in real-time PCR in negative control? - (reply: 1)
-
Problem with QPCR - control gene does not amplify - (reply: 2)
-
qPCR for HKG and traditional PCR for GOI - (reply: 1)
-
Smeary bands in real-time RT-PCR - (reply: 7)
-
Touch down real-time PCR? - (reply: 1)
-
real-time slopes - (reply: 4)
-
RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
-
1st time real-time PCR questions - (reply: 1)
-
real-time pcr optimization problem - (reply: 4)
-
Optimal size for qRt-PCR - (reply: 3)
-
Primer Dimer problems in real-time PCR - (reply: 14)
-
Has anyone used 8E5 cells as standard for HIV qPCR? - HIV standards (reply: 1)
-
some qPCR questions (naive ones included)... - first pitfals etc... (reply: 8)
-
designing QRT-PCR Primers - (reply: 11)
-
Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
-
Another QPCR problem. Please help - (reply: 5)
-
Trouble with standard curves for real-time PCR - (reply: 6)
-
Real-time PCR machines for DNA quantitation - (reply: 5)
-
qPCR after ChIP - lack of reproducibility - (reply: 9)
-
Use of ROX in qPCR - (reply: 8)
-
strange QPCR amplificaton plot - (reply: 3)
-
total RNA from BSE infected WBC for microarray and qPCR - (reply: 2)
-
Mg curve for real-time PCR - Strange result (reply: 5)
-
Northern vs qRT-PCR for expression quantitation - (reply: 6)
-
Real-time PCR probe degradation - when do they degrades? (reply: 4)
-
My SYBRgreen qPCR stopped working - (reply: 3)
-
AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
-
real-time thresholds - purpose? - (reply: 1)
-
Real-time PCR - contamination or not? - (reply: 6)
-
real-time PCR - Multiple melting curve peaks (reply: 18)
-
Real-time PCR questions (Mx3000P) - Need some help with interpretation (reply: 7)
-
QPCR books - (reply: 1)
-
qRT-PCR data analysis - (reply: 1)
-
qPCR and siRNA - (reply: 2)
-
mastermix for Real-time PCR - (reply: 2)
-
Real-Time qRT-PCR standard curves... efficiency is too high! - (reply: 19)
-
qPCR standard curves - how can I keep the quality good? (reply: 6)
-
Construct DNA plasmid for QPCR - (reply: 4)
-
real-time PCR - (reply: 5)
-
Real-time PCR amplification curve up and down - (reply: 3)
-
Which RT primer is better for qPCR - (reply: 2)
-
Do I have amplication of RNA by qPCR? - (reply: 7)
-
melt curves for SYBR Green real-time PCR driving me nuts!! - melt curves real-time PCR (reply: 6)
-
real-time RT-PCR one cell - (reply: 2)
-
real-time pcr plasmid standards - (reply: 4)
-
QRT-PCR - (reply: 4)
-
contamination in real-time PCR - (reply: 32)
-
Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
-
contamination in real-time pcr negative control - (reply: 12)
-
Real-time PCR problems - (reply: 3)
-
real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
-
Real-time PCR and high Ct value - (reply: 1)
-
product size for real-time PCR primer - (reply: 3)
-
Efficiency Real-Time PCR - Real-time PCR (reply: 3)
-
Real-time quantification in Chromatin IP - (reply: 2)
-
qPCR on genomic DNA - (reply: 2)
-
Real-Time PCR - No plateau! (reply: 1)
-
qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
-
does anyone know real-time methyaltion pcr? - (reply: 1)
-
real-time PCR - How to design primers (reply: 2)
-
About Q-PCR(real-time PCR) - COMPARE (reply: 1)
-
QRT-PCR result analysis - relative transcript level? (reply: 3)
-
Familiar with MX3000P by Stratagene?? - qPCR (reply: 1)
-
Probes for Real-Time PCR - (reply: 3)