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Mutagenesis Related Discussions
heterozygous missense mutation -
(reply: 2)
mutant identify - help
(reply: 2)
Site-directed mutagenesis -
(reply: 12)
mutational studies -
(reply: 4)
Pioneers of Mutagenesis to Probe Protein Structure and Function -
(reply: 1)
Mutagenesis - To amplify fragments for mutagenesis
(reply: 1)
No mutation with mutagenesis kit -
(reply: 1)
Is Crossover a form of mutation? - Crossover Mutation
(reply: 1)
targeted random mutagenesis gave majorly CCCs -
(reply: 1)
How to identify whether a mutation in intron is binding site? -
(reply: 1)
Problems with site-directed mutagenesis -
(reply: 2)
get of ura3 mutant of a yeast -
(reply: 1)
kras mutation. best method for detection -
(reply: 4)
will site directed mutagenesis work for 8kb construct? -
(reply: 4)
troubleshooting with site directed mutagenesis -
(reply: 2)
Point Mutation, Protein Folding, Base Pairing -
(reply: 3)
No mRNA or protein made by my mutant gene -
(reply: 4)
Knock -in & mutation experiments - Knock -in & mutation experiments
(reply: 2)
High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid
(reply: 1)
Single nucleotide polymorphism vs single nucleotide mutation -
(reply: 4)
Dh5alpha for site directed mutagenesis -
(reply: 3)
high melting temperature in site directed mutagenesis -
(reply: 4)
problem with site mutation -
(reply: 5)
How to construct a Double mutant -
(reply: 7)
Dominant Negative Mutant -
(reply: 3)
Problems with site directed mutagenesis -
(reply: 3)
QuickChange II Site-Directed Mutagenesis Kit Stratagene -
(reply: 7)
point mutation -
(reply: 8)
How to create a mutant DNA having point mutations sequence? -
(reply: 1)
Deletion mutant protein prediction -
(reply: 3)
Problems after site directed mutagenesis -
(reply: 5)
site mutation problem -
(reply: 2)
QuickChnage Mutagenesis problem - no mutation, please help!!!!!!!!
(reply: 14)
Can you get two proteins made from a heterozygous gene mutation? -
(reply: 3)
Site-Directed Mutagenesis Kit -
(reply: 1)
primer Tm for PCR mutagenesis -
(reply: 1)
Zebrafish Mutant -
(reply: 2)
Polymerase for Quikchange mutagenesis -
(reply: 3)
Quikchange mutagenesis primer and PCR design -
(reply: 3)
Recommendations for a good site-directed mutagenesis kit -
(reply: 3)
Mutagenesis PCR transforming into BL21(DE3) -
(reply: 2)
Mutagenesis and primer purity? -
(reply: 4)
site directed mutagenesis using synthesized oligos -
(reply: 1)
mutagenesis of 6 bases - what is the best option?
(reply: 1)
Anyone that had introduced a nt with QuikChange mutagenesis kit? -
(reply: 3)
site directed mutagenesis problem - Getting no colonies
(reply: 5)
What do you think is the best mutagenesis strategy if you want to mutate several -
(reply: 4)
Site-directed mutagenesis problem - Mutations under oligo
(reply: 2)
QuikChange™ Site-Directed Mutagenesis Kit -
(reply: 4)
random mutation during mutagenesis - will all colonies on LB plate have the same mutation?
(reply: 3)
primer design for site-directed mutagenesis -
(reply: 3)
Mutation Screening - DNA Sequences... -
(reply: 3)
problems with mutant PCR - problems with mutant PCR
(reply: 1)
mutation by gamma ray - why gamma ray?
(reply: 3)
mutation on ficus deltoidea - any experience to share?
(reply: 3)
How to quantify wild-type and mutant protein? -
(reply: 4)
How do I correct a frameshift mutation? -
(reply: 2)
mutation and glycerol stock -
(reply: 10)
anybody using GalK cassette for BAC mutation? -
(reply: 5)
mutant PCR -
(reply: 4)
Induce mutation in my gene -
(reply: 5)
Mutation in plasmid -
(reply: 2)
cryptic mutation -
(reply: 4)
pcr mutagenesis -
(reply: 4)
Site-directed mutagenesis -
(reply: 6)
mutation in a gene that is not expressing a protein -
(reply: 4)
Why are CpG sites more prone to mutation? -
(reply: 4)
Site directed mutagenesis on Human GST T1-1 - Any advice for an SDM n00b?
(reply: 1)
Trouble sequencing DNA from mutagenesis reaction -
(reply: 1)
mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's
(reply: 6)
Point Mutation in DNA - Quik change mutagenesis
(reply: 1)
sitedirected mutagenesis -
(reply: 4)
Guessing null mutation -
(reply: 6)
Codons particularly vunerable to mutation? -
(reply: 3)
Site Directed Mutagenesis Reaction! -
(reply: 1)
Invitrogen GeneTailor Site-Directed Mutagenesis Reaction--HELP! -
(reply: 1)
Quickchange site directed mutagenesis/PCR problem - Transformation isn't working :(
(reply: 8)
how would you detect a point mutation in the genome? PCR? -
(reply: 3)
biological mutagenesis -
(reply: 2)
Promega :Mutagenesis Systems -
(reply: 5)
Low mutation efficiency -
(reply: 7)
site-directed mutagenesis of lysine -
(reply: 2)
Generating homologous mutant stem cells - any stricky point?
(reply: 2)
Site-Directed Mutagenesis (megaprimer) of internal sequence -
(reply: 5)
K to R mutation for kinase activity change -
(reply: 1)
southern conflicts with PCR in knock-in mouse screening - why southern positives are not the mutant we want
(reply: 5)
Mutation -
(reply: 7)
TA cloning and mutation -
(reply: 3)
Pfx polymerase for site-directed mutagenesis -
(reply: 2)
mutagenesis in Enterococcus spp -
(reply: 2)
Chances of Mutation -
(reply: 3)
Mutagenesis not working - no colonies
(reply: 15)
about mutant plasmid construction -
(reply: 3)
How can I make a point mutation -
(reply: 4)
Site Directed Mutagenesis within CpG rich promoter -
(reply: 4)
Problem in Patch PCR for Site-Directed Mutagenesis -
(reply: 5)
Mutagenesis Help (plasmid isolation & sequencing stages) -
(reply: 2)
mutation during transformation? -
(reply: 3)
Strange Maxi-Prep problem - low yields with QuickChange mutagenesis competent E. Coli
(reply: 1)
HA tag vs His(6) tag , with mutagenesis -
(reply: 6)
good program to model 3D changes to protein from mutation -
(reply: 1)
site directed mutagenesis stratagene philosophy - PCR prob
(reply: 5)
Mutagenesis using 4 sets of primer -
(reply: 6)
Site-directed Mutagenesis -
(reply: 3)
Site-directed Mutagenesis -
(reply: 22)
problems with expression of mutant protein -
(reply: 1)
Mutation Vs polymorphism - Mutation Vs Polymorphism
(reply: 5)
Mutation Vs polymorphism - Mutation Vs polymorphism
(reply: 7)
Dominant negative mutation: Tyr is usually mutate to ? - Site directed mutation
(reply: 1)
Protein and Proteomics - Point mutation and protein stability
(reply: 2)
virus mutation rate monitor -
(reply: 1)
overall strucure change - amino acid mutation
(reply: 1)
primers design for mutagenesis of two nearby sites - mutations
(reply: 2)
Join GFP to my insert without mutagenesis -
(reply: 8)
Mutagenesis to make 45bp deletion -
(reply: 1)
How to get a mutant in Streptococcus aglactiae -
(reply: 3)
can you introduce mutation directly into the cells? - other than just transfecting it with a vector...
(reply: 1)
isolation of DNA from a plasmid for site-directed mutagenesis? - do researchers actually do this?
(reply: 6)
QuickChange mutagenesis: how to do it without kit? - What dNTP concentration should I use?
(reply: 1)
Side directed mutagenesis with 17kb plasmid -
(reply: 19)
How many mismatches in point mutation primers? -
(reply: 3)
how to mutant the gene on the genomic DNA? -
(reply: 9)
Site directed mutagenesis GFP to RFP -
(reply: 4)
Can I make four substitutions (mutagenesis) using PCR at a time? - help me out, pls :)
(reply: 7)
How to make a point mutation in a wild type plasmid. -
(reply: 2)
Testing for binding of mutant proteins to cell surface receptors -
(reply: 3)
Proof Reading Taq Polymerase - How effective can it be in mutation study
(reply: 2)
Directional cloning and clone mutations - each clone has a different mutation!
(reply: 1)
Site directed mutagenesis - his tag - insertional mutations
(reply: 6)
site directed mutagenesis - point mutagenesis of target gene
(reply: 3)
How does sodium thiosulphate affect yeast?? - Yeast mutagenesis
(reply: 1)
PCR MUTAGENESIS PROBLEM - SiteDirected Mutagenesis - Problem
(reply: 3)
site-directed mutagenesis -
(reply: 10)
Difference between SNP and point mutation -
(reply: 12)
site directed mutagenesis -
(reply: 5)
Site Directed Mutagenesis Problem -
(reply: 5)
site directed mutagenesis -
(reply: 4)
Rate of spontaneous mutation in yeast - Yeast
(reply: 2)
Transposon mutagenesis of Pseudomonas with Epicentre kit - Need a method for identifying the disrupted gene
(reply: 1)
Plasmid gene mutation -
(reply: 1)
sucide plasmid directed mutagenesis - sucide plasmid directed mutagenesis in pseudomonas
(reply: 1)
a question about mutagenesis -
(reply: 5)
Calculation of Mutation Rate - Calculation of Mutation Rate
(reply: 2)
mutation in cancer cell line -
(reply: 1)
Chemical mutagenesis of plasmids - Why only hydroxylamine ?
(reply: 3)
URGENT...site directed mutagenesis -
(reply: 4)
pcr mutagenesis -
(reply: 3)
PCR mutagenesis -
(reply: 1)
Mutagenesis - How to use quick change method -
(reply: 1)
Mutagenesis -
(reply: 1)
Mutational Event, Wild type enzyme Sequence -
(reply: 2)
Site-directed Mutagenesis using Dut-Ung system - Troubleshooting a mutagenesis
(reply: 1)
mutagenesis -
(reply: 1)