How does sodium thiosulphate affect yeast?? - Yeast mutagenesis (Jul/27/2005 )

Hi there!!

I've been doing some yeast mutagenesis using EMS followed by quenching with 5% sodium thiosulphate.

However, when we culture these mutated guys, they come out rather smelly (sulphorous smell, probably from the quenching agent). But I was wondering if anyone knew if this agent had an effect on yeast growth or particularly on the yeast cell wall balance/composition?! I've tried getting rid of the quenching agent by centrifugation, but the yeast don't appear to pellet easily.

Any tips or helpful facts would be muchly appreciated!!

Ciao for now,

Ritchie

hi
a colleague uses this protocol and it works well for him. The survivability of the treated yeast is quite little less as untreted but his exp are ok.
fred