Dominant Negative Mutant - (Feb/20/2008 )
Can some one explain me the concept of dominant negative mutant
-samita-
A dominant-negative mutation usually means that the resulting protein is has lost a certain part of its function (negative), but it can out-compete the endogenous protein in some way (dominant).
For example:
Say you have protein receptor X. Frequently, a dominant-negative will still possess the extra-cellular ligand binding domain and the transmembrane domain, but it will lack a cytoplasmic domain that is required to interact with down-stream proteins that are required to transmit the signal.
So the DN version of X can sequester ligands from endogenous receptor X, leading to a partial loss-of-function
-SLepage-
QUOTE (SLepage @ Feb 20 2008, 09:01 AM)
A dominant-negative mutation usually means that the resulting protein is has lost a certain part of its function (negative), but it can out-compete the endogenous protein in some way (dominant).
For example:
Say you have protein receptor X. Frequently, a dominant-negative will still possess the extra-cellular ligand binding domain and the transmembrane domain, but it will lack a cytoplasmic domain that is required to interact with down-stream proteins that are required to transmit the signal.
So the DN version of X can sequester ligands from endogenous receptor X, leading to a partial loss-of-function
For example:
Say you have protein receptor X. Frequently, a dominant-negative will still possess the extra-cellular ligand binding domain and the transmembrane domain, but it will lack a cytoplasmic domain that is required to interact with down-stream proteins that are required to transmit the signal.
So the DN version of X can sequester ligands from endogenous receptor X, leading to a partial loss-of-function
you are right SLepage but your example explains an inactive ("negative") form, the aspect of dominance, I think, is missing
-The Bearer-
thanks a lot for your explanation. 
QUOTE (SLepage @ Feb 20 2008, 09:01 AM)
A dominant-negative mutation usually means that the resulting protein is has lost a certain part of its function (negative), but it can out-compete the endogenous protein in some way (dominant).
For example:
Say you have protein receptor X. Frequently, a dominant-negative will still possess the extra-cellular ligand binding domain and the transmembrane domain, but it will lack a cytoplasmic domain that is required to interact with down-stream proteins that are required to transmit the signal.
So the DN version of X can sequester ligands from endogenous receptor X, leading to a partial loss-of-function
For example:
Say you have protein receptor X. Frequently, a dominant-negative will still possess the extra-cellular ligand binding domain and the transmembrane domain, but it will lack a cytoplasmic domain that is required to interact with down-stream proteins that are required to transmit the signal.
So the DN version of X can sequester ligands from endogenous receptor X, leading to a partial loss-of-function
-samita-