Knock -in & mutation experiments - Knock -in & mutation experiments (Jun/10/2008 )
Hi guys ,
I am looking for some help into the following :
I have a cell line which is a knockout for a cell-cycle gene , i want to knock -in that gene in to my cells plus i want to also make my cell line heterzygous for that gene. How should i go about this .
I have another query , after being able to do the stuff mentioned above....in my knock -in and heterozygous cell line that i will obtain , i want to mutate another gene . Is all this possible and how should i go about . Please elaborate as i have no experience with this stuff.
Thanks a lot 
-seashell83-
QUOTE (seashell83 @ Jun 10 2008, 01:28 PM)
Hi guys ,
I am looking for some help into the following :
I have a cell line which is a knockout for a cell-cycle gene , i want to knock -in that gene in to my cells plus i want to also make my cell line heterzygous for that gene. How should i go about this .
I have another query , after being able to do the stuff mentioned above....in my knock -in and heterozygous cell line that i will obtain , i want to mutate another gene . Is all this possible and how should i go about . Please elaborate as i have no experience with this stuff.
Thanks a lot
I am looking for some help into the following :
I have a cell line which is a knockout for a cell-cycle gene , i want to knock -in that gene in to my cells plus i want to also make my cell line heterzygous for that gene. How should i go about this .
I have another query , after being able to do the stuff mentioned above....in my knock -in and heterozygous cell line that i will obtain , i want to mutate another gene . Is all this possible and how should i go about . Please elaborate as i have no experience with this stuff.
Thanks a lot
Is this the same cell line?
-cellcounter-
QUOTE (cellcounter @ Jun 10 2008, 06:03 PM)
QUOTE (seashell83 @ Jun 10 2008, 01:28 PM)
Hi guys ,
I am looking for some help into the following :
I have a cell line which is a knockout for a cell-cycle gene , i want to knock -in that gene in to my cells plus i want to also make my cell line heterzygous for that gene. How should i go about this .
I have another query , after being able to do the stuff mentioned above....in my knock -in and heterozygous cell line that i will obtain , i want to mutate another gene . Is all this possible and how should i go about . Please elaborate as i have no experience with this stuff.
Thanks a lot
I am looking for some help into the following :
I have a cell line which is a knockout for a cell-cycle gene , i want to knock -in that gene in to my cells plus i want to also make my cell line heterzygous for that gene. How should i go about this .
I have another query , after being able to do the stuff mentioned above....in my knock -in and heterozygous cell line that i will obtain , i want to mutate another gene . Is all this possible and how should i go about . Please elaborate as i have no experience with this stuff.
Thanks a lot
Is this the same cell line?
Yep i want to do it in the same cell line , to keep the rest of the background same .
-seashell83-