Inclusion Body purification - (Feb/23/2010 )

Hello,

I have two recombinant proteins that are expressed as inclusion bodies and when I try to purify these inclusion bodies I obtain many contaminants. I have been using a buffer composed by sodium phosphate 20 mM, NaCl 500mM and Imidazole 10 mM, followed by lisozyme (1mg/mL) and sonication, in bacterial lysis process. Then I use the same lysis buffer + Urea 8M to denaturate my protein. Does anybody know how can I obatin inclusion bodies with high purity?
Thanks very much!

Please go through the attached paper (highlighted text) for purification of protein from IBs.


Hope this helps.

Best regards,
Kaushik

KAUSHIK THAKKAR on Feb 24 2010, 06:12 AM said:

Please go through the attached paper (highlighted text) for purification of protein from IBs.


Hope this helps.

Best regards,
Kaushik