kinase - kinase assay (Oct/05/2007 )

does anyone use noradioactif kinase assay?

I have my subtrate and want to pull down my kinase et check the activity.

do you know how long the subtstrat and kinase are in contact?
what I meant is it it possible to show the interactoin between them by TH, copIP or FRET?

thanks

ulujm

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I do not know what TH means; for co-IP you need anti-phospho-specifc antibodies; FRET sounds always good but needs some runs to get reliable results...

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YES, FRET is a suitable good technique to determine protein-protein interaction. You can try two-hybrid assay too

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