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31. RNAi of non-coding RNAs....? - (reply: 6)
32. Invitrogen's Negative control siRNA - (reply: 3)
33. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
34. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
35. Gene Knockout - (reply: 2)
36. Dissolve siRNA in non-RNase free buffer - (reply: 1)
37. Spacing of artificial miRNA target sites for efficient knockdown - (reply: 1)
38. miRNA 3' end nucleotidylation - (reply: 4)
39. CD4 siRNA - (reply: 1)
40. Inducible Lentiviral systems - (reply: 1)
41. gene dowregulation by miRNA - (reply: 1)
42. SMP30 silencing in vivo - stem cell biology (reply: 2)
43. How to construct miRNA expression vector - (reply: 1)
44. Discovery of novel miRNAs - (reply: 1)
45. miRNA profiling - (reply: 6)
46. Optimization of siRNA experiments - (reply: 1)
47. optimum time for assay of target gene - siRNA (reply: 1)
48. Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES? (reply: 3)
49. transcriptome? - (reply: 3)
50. Xho1/Bglll digest of pSUPER.retro.puro with stuffer problems - (reply: 1)
51. miRNA introns and exons - (reply: 1)
52. poorly conserved miRNA targets - conserved vs pooly conserved targets (reply: 1)
53. miRNA and MRE difference - (reply: 6)
54. Confusion about proper endogenous control for microRNAs - why miR-16? so confused... (reply: 3)
55. different sirna-different henotypes, which one is rEAL? - (reply: 1)
56. miRNA poly A tailing - (reply: 2)
57. siRNA toxicity in MCF7 cells - why are they sick????? (reply: 1)
58. miRNA family - (reply: 2)
59. Transformation didnt work - (reply: 2)
60. miRNA seed - understanding 5'UTR and 3'UTR (reply: 7)
32. Invitrogen's Negative control siRNA - (reply: 3)
33. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
34. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
35. Gene Knockout - (reply: 2)
36. Dissolve siRNA in non-RNase free buffer - (reply: 1)
37. Spacing of artificial miRNA target sites for efficient knockdown - (reply: 1)
38. miRNA 3' end nucleotidylation - (reply: 4)
39. CD4 siRNA - (reply: 1)
40. Inducible Lentiviral systems - (reply: 1)
41. gene dowregulation by miRNA - (reply: 1)
42. SMP30 silencing in vivo - stem cell biology (reply: 2)
43. How to construct miRNA expression vector - (reply: 1)
44. Discovery of novel miRNAs - (reply: 1)
45. miRNA profiling - (reply: 6)
46. Optimization of siRNA experiments - (reply: 1)
47. optimum time for assay of target gene - siRNA (reply: 1)
48. Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES? (reply: 3)
49. transcriptome? - (reply: 3)
50. Xho1/Bglll digest of pSUPER.retro.puro with stuffer problems - (reply: 1)
51. miRNA introns and exons - (reply: 1)
52. poorly conserved miRNA targets - conserved vs pooly conserved targets (reply: 1)
53. miRNA and MRE difference - (reply: 6)
54. Confusion about proper endogenous control for microRNAs - why miR-16? so confused... (reply: 3)
55. different sirna-different henotypes, which one is rEAL? - (reply: 1)
56. miRNA poly A tailing - (reply: 2)
57. siRNA toxicity in MCF7 cells - why are they sick????? (reply: 1)
58. miRNA family - (reply: 2)
59. Transformation didnt work - (reply: 2)
60. miRNA seed - understanding 5'UTR and 3'UTR (reply: 7)