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271. How to detect dsRNA? - (reply: 3)
272. Knockdown detected by western but not RT-PCR - (reply: 3)
273. shRNA lenti vectors - (reply: 5)
274. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
275. miRNA ladder problem - (reply: 5)
276. Trouble with cloning specific miRNA and mRNA 3' termini? - (reply: 3)
277. shRNA versus siRNA - (reply: 6)
278. shRNA system - (reply: 2)
279. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
280. Normalization-mirRNAs - (reply: 2)
281. inducible shRNA promoter leaking - (reply: 1)
282. GFP siRNA transfection - readout - (reply: 5)
283. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
284. pri-miRNA transcript - get the exact primary transcripts of miRNAs (reply: 4)
285. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
286. siRNA protocol in floating cells - (reply: 1)
287. miRNA single or double stranded? - (reply: 11)
288. Detect small RNA using DIG-labeled probe - Northern for small RNA (reply: 3)
289. siRNA transfection problem - (reply: 4)
290. how to know/predict a specific gene is regulated by miRNA - (reply: 7)
291. siRNA transfection reagent - (reply: 9)
292. siRNA and Hybond XL - any one use them? (reply: 1)
293. beginners guide to stable RNAi transfection - (reply: 1)
294. Retroviral vs. Lentiviral packaging - Are the packaging signals compatible? (reply: 1)
295. siRNA: how long will knock-down last - (reply: 6)
296. miRNA northern protocol - (reply: 3)
297. miRNA targets in comparison to siRNA targets - (reply: 2)
298. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
299. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
300. RNAse Protection Assay for microRNA - is anyone using the mirVana Detection Kit? (reply: 2)
272. Knockdown detected by western but not RT-PCR - (reply: 3)
273. shRNA lenti vectors - (reply: 5)
274. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
275. miRNA ladder problem - (reply: 5)
276. Trouble with cloning specific miRNA and mRNA 3' termini? - (reply: 3)
277. shRNA versus siRNA - (reply: 6)
278. shRNA system - (reply: 2)
279. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
280. Normalization-mirRNAs - (reply: 2)
281. inducible shRNA promoter leaking - (reply: 1)
282. GFP siRNA transfection - readout - (reply: 5)
283. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
284. pri-miRNA transcript - get the exact primary transcripts of miRNAs (reply: 4)
285. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
286. siRNA protocol in floating cells - (reply: 1)
287. miRNA single or double stranded? - (reply: 11)
288. Detect small RNA using DIG-labeled probe - Northern for small RNA (reply: 3)
289. siRNA transfection problem - (reply: 4)
290. how to know/predict a specific gene is regulated by miRNA - (reply: 7)
291. siRNA transfection reagent - (reply: 9)
292. siRNA and Hybond XL - any one use them? (reply: 1)
293. beginners guide to stable RNAi transfection - (reply: 1)
294. Retroviral vs. Lentiviral packaging - Are the packaging signals compatible? (reply: 1)
295. siRNA: how long will knock-down last - (reply: 6)
296. miRNA northern protocol - (reply: 3)
297. miRNA targets in comparison to siRNA targets - (reply: 2)
298. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
299. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
300. RNAse Protection Assay for microRNA - is anyone using the mirVana Detection Kit? (reply: 2)