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421. Lentiviral vector production timeline - (reply: 1)
422. Ambion low conductivity electroporation buffer? - (reply: 1)
423. Transfecting NIH 3T3 cells - looking for effiency for maximum knockdown (reply: 4)
424. Isolate tRNA from total RNA - (reply: 2)
425. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
426. co-transfecting DNA and RNA duplexes - (reply: 3)
427. transfection or injection of siRNA - Which one is better? (reply: 9)
428. about siRNA and shRNA - RNAi Vector (reply: 2)
429. siRNA in vitro transcription-Ambion kit? - (reply: 3)
430. Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
431. siRNA to shRNA problem - (reply: 7)
432. direct transfection vs. retroviral transfection - (reply: 2)
433. Question about doing RNAi in primary cells - (reply: 3)
434. Knockdown protein expression using rnai - (reply: 4)
435. Isolating RNA from a small number of cells - (reply: 1)
436. Detecting DICER by Westen blotting - (reply: 2)
437. micro RNA traget prediction - (reply: 5)
438. Lipofectimine? How to optomize?Some basic Questions? - (reply: 4)
439. Lineaerization of pENTR/H1/TO? - (reply: 1)
440. I ran out of transfection agent...What to do? - (reply: 3)
441. cellular death during dsRNA transfection. - (reply: 3)
442. How to extract siRNA from primordial leaf - (reply: 1)
443. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
444. what is the difference about siRAN, miRNA, shRNA and RNAi - (reply: 1)
445. how do I get the source code for miranda - miranda (reply: 2)
446. RNA pol II driven shRNA expression - CMV driven shRNA expression (reply: 10)
447. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
448. Basic fundamental principles of gene knockout - (reply: 2)
449. Basic questions about siRNA - (reply: 6)
450. siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)
422. Ambion low conductivity electroporation buffer? - (reply: 1)
423. Transfecting NIH 3T3 cells - looking for effiency for maximum knockdown (reply: 4)
424. Isolate tRNA from total RNA - (reply: 2)
425. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
426. co-transfecting DNA and RNA duplexes - (reply: 3)
427. transfection or injection of siRNA - Which one is better? (reply: 9)
428. about siRNA and shRNA - RNAi Vector (reply: 2)
429. siRNA in vitro transcription-Ambion kit? - (reply: 3)
430. Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
431. siRNA to shRNA problem - (reply: 7)
432. direct transfection vs. retroviral transfection - (reply: 2)
433. Question about doing RNAi in primary cells - (reply: 3)
434. Knockdown protein expression using rnai - (reply: 4)
435. Isolating RNA from a small number of cells - (reply: 1)
436. Detecting DICER by Westen blotting - (reply: 2)
437. micro RNA traget prediction - (reply: 5)
438. Lipofectimine? How to optomize?Some basic Questions? - (reply: 4)
439. Lineaerization of pENTR/H1/TO? - (reply: 1)
440. I ran out of transfection agent...What to do? - (reply: 3)
441. cellular death during dsRNA transfection. - (reply: 3)
442. How to extract siRNA from primordial leaf - (reply: 1)
443. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
444. what is the difference about siRAN, miRNA, shRNA and RNAi - (reply: 1)
445. how do I get the source code for miranda - miranda (reply: 2)
446. RNA pol II driven shRNA expression - CMV driven shRNA expression (reply: 10)
447. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
448. Basic fundamental principles of gene knockout - (reply: 2)
449. Basic questions about siRNA - (reply: 6)
450. siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)