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1. discuss about northern for miRNAs - northern, miRNA (reply: 1)
2. how to predict pre-miRNA and target - (reply: 2)
3. Just ordered 100 euros worth of kit that apparently doesnt work well! - Help!!!! PhD student on the verge of......being fired maybe (reply: 3)
4. Designing shRNA - (reply: 2)
5. Naming of miRNA - (reply: 2)
6. miRNA expression vector - (reply: 3)
7. siRNA modification to make it more hydrophobic - (reply: 1)
8. microRNA in situ hybridization - label probe 5' or 3'? (reply: 1)
9. knocking down endogeous gene using shRNA - (reply: 6)
10. Validation of MicroRNA array results - (reply: 1)
11. definition of miRNA family - (reply: 1)
12. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
13. downregulation of GATA 3 by shRNA's - (reply: 1)
14. RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
15. siRNA designation for different genes - (reply: 1)
16. Exiqon miRNA arrays- any experiences - difficulties in validation (reply: 4)
17. Transfected copies per cell - (reply: 1)
18. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
19. naked shRNA transfection - (reply: 2)
20. Double SiRNA trasfection - (reply: 1)
21. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
22. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
23. mirvana isolation kit - (reply: 5)
24. confusion about off-target effect - (reply: 3)
25. Why do miRNAs always bind to 3'UTR? - (reply: 3)
26. Sirna concentration - (reply: 1)
27. suggested siRNA concentrations - (reply: 1)
28. RNAi of non-coding RNAs....? - (reply: 6)
29. Invitrogen's Negative control siRNA - (reply: 3)
30. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
2. how to predict pre-miRNA and target - (reply: 2)
3. Just ordered 100 euros worth of kit that apparently doesnt work well! - Help!!!! PhD student on the verge of......being fired maybe (reply: 3)
4. Designing shRNA - (reply: 2)
5. Naming of miRNA - (reply: 2)
6. miRNA expression vector - (reply: 3)
7. siRNA modification to make it more hydrophobic - (reply: 1)
8. microRNA in situ hybridization - label probe 5' or 3'? (reply: 1)
9. knocking down endogeous gene using shRNA - (reply: 6)
10. Validation of MicroRNA array results - (reply: 1)
11. definition of miRNA family - (reply: 1)
12. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
13. downregulation of GATA 3 by shRNA's - (reply: 1)
14. RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
15. siRNA designation for different genes - (reply: 1)
16. Exiqon miRNA arrays- any experiences - difficulties in validation (reply: 4)
17. Transfected copies per cell - (reply: 1)
18. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
19. naked shRNA transfection - (reply: 2)
20. Double SiRNA trasfection - (reply: 1)
21. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
22. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
23. mirvana isolation kit - (reply: 5)
24. confusion about off-target effect - (reply: 3)
25. Why do miRNAs always bind to 3'UTR? - (reply: 3)
26. Sirna concentration - (reply: 1)
27. suggested siRNA concentrations - (reply: 1)
28. RNAi of non-coding RNAs....? - (reply: 6)
29. Invitrogen's Negative control siRNA - (reply: 3)
30. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)