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301. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
302. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
303. RNAse Protection Assay for microRNA - is anyone using the mirVana Detection Kit? (reply: 2)
304. use genomic DNA or cDNA? - (reply: 1)
305. Basic question about siRNA, shRNA..etc - What's the difference? (reply: 5)
306. one Step RT with Sybr and standard curve - (reply: 1)
307. miRNA extraction from primary tumor samples - (reply: 2)
308. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
309. Expression in other mammalian cells with human H1 promoter - (reply: 1)
310. Biosafety Level of shRNA Lentiviral Transduction Particles - (reply: 1)
311. miRNA promoter ? - how to discover MicroRNA promoter element (reply: 1)
312. miRNA quantification - easiest/cheapest method of measuring expression of a single miRNA in differnt ce (reply: 3)
313. Sources for vector-based siRNA against kinases? - (reply: 1)
314. About the conc. use of the siRNA - (reply: 4)
315. about using the pLentiLOX 3.7 vector in the lentiviral system - (reply: 6)
316. Is RNAi patented? - (reply: 4)
317. miRNA inhibitors - 2'-O-methyl versus Ambion antisense oligos (reply: 4)
318. How to design the loop for shRNA? - (reply: 2)
319. does anyone knows a working shrna for murine Caspase-11 - (reply: 1)
320. Contaminated Duplex - how to clean it up.. (reply: 1)
321. splice variants and knock down - (reply: 2)
322. Express miRNA in vector for gene knockdown - (reply: 4)
323. design artificial miRNA target - (reply: 3)
324. is there any fast screening after siRNA knockdown of protein? - (reply: 3)
325. siRNA and mices - What is the best way to in vivo siRNA transfer (reply: 6)
326. RealTime PCR of microRNAs - (reply: 9)
327. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
328. How to overexpress miRNA? - (reply: 6)
329. How to amplify intronic antisense transcript? - (reply: 3)
330. shRNA Plasmid propagation in Xl1 Blue - (reply: 1)
302. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
303. RNAse Protection Assay for microRNA - is anyone using the mirVana Detection Kit? (reply: 2)
304. use genomic DNA or cDNA? - (reply: 1)
305. Basic question about siRNA, shRNA..etc - What's the difference? (reply: 5)
306. one Step RT with Sybr and standard curve - (reply: 1)
307. miRNA extraction from primary tumor samples - (reply: 2)
308. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
309. Expression in other mammalian cells with human H1 promoter - (reply: 1)
310. Biosafety Level of shRNA Lentiviral Transduction Particles - (reply: 1)
311. miRNA promoter ? - how to discover MicroRNA promoter element (reply: 1)
312. miRNA quantification - easiest/cheapest method of measuring expression of a single miRNA in differnt ce (reply: 3)
313. Sources for vector-based siRNA against kinases? - (reply: 1)
314. About the conc. use of the siRNA - (reply: 4)
315. about using the pLentiLOX 3.7 vector in the lentiviral system - (reply: 6)
316. Is RNAi patented? - (reply: 4)
317. miRNA inhibitors - 2'-O-methyl versus Ambion antisense oligos (reply: 4)
318. How to design the loop for shRNA? - (reply: 2)
319. does anyone knows a working shrna for murine Caspase-11 - (reply: 1)
320. Contaminated Duplex - how to clean it up.. (reply: 1)
321. splice variants and knock down - (reply: 2)
322. Express miRNA in vector for gene knockdown - (reply: 4)
323. design artificial miRNA target - (reply: 3)
324. is there any fast screening after siRNA knockdown of protein? - (reply: 3)
325. siRNA and mices - What is the best way to in vivo siRNA transfer (reply: 6)
326. RealTime PCR of microRNAs - (reply: 9)
327. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
328. How to overexpress miRNA? - (reply: 6)
329. How to amplify intronic antisense transcript? - (reply: 3)
330. shRNA Plasmid propagation in Xl1 Blue - (reply: 1)