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211. Paper on miRNA knockdowns - Using Morpholinos for knockdown & specificity controls (reply: 1)
212. the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
213. miRNA luciferase reporter assay queries - (reply: 1)
214. Plant miRNA--Need your help! - (reply: 1)
215. 5' adaptor ligation fail - (reply: 3)
216. miRNA expression vector - miRNA expression vector (reply: 4)
217. Dual luciferase assay - (reply: 2)
218. Problem with gfp express in S2 - (reply: 7)
219. 3' terminal post-transcriptional processing of miRNAs? - (reply: 2)
220. Double - Triple stable transfection - (reply: 4)
221. Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
222. qPCR for microRNAs - minimal primer length? (reply: 6)
223. mRNA induction after shRNA transfection - (reply: 3)
224. Loss of RNAi after changing culture media? - (reply: 2)
225. Dicer knockdown in mice - (reply: 1)
226. transfection agent -primary mouse macrophages, anybody? - (reply: 1)
227. cloning shRNA into pLentilox 3.7 - (reply: 3)
228. validated siRNA doesn't work - (reply: 6)
229. protein-protein interaction-plz help - (reply: 12)
230. Trouble cloning miRNAs - (reply: 5)
231. RNAi - vector construction (reply: 3)
232. polyA tail as 3' adaptor to clone miRNA - (reply: 1)
233. need protocol for precipitation of 13 mer mRNA - (reply: 3)
234. Has any one successfully performed miRNA cloning? - miRNA cloning troubleshooting (reply: 5)
235. sirna half life - time of effect (reply: 2)
236. Uridines in linker design for miRNA cloning. - Why uridine is often used in the 3ยด-Linkers. (reply: 2)
237. RNAase-free saline - (reply: 1)
238. shRNA control - (reply: 3)
239. Biotin ou HRP - (reply: 1)
240. transfection of chick cell - (reply: 3)
212. the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
213. miRNA luciferase reporter assay queries - (reply: 1)
214. Plant miRNA--Need your help! - (reply: 1)
215. 5' adaptor ligation fail - (reply: 3)
216. miRNA expression vector - miRNA expression vector (reply: 4)
217. Dual luciferase assay - (reply: 2)
218. Problem with gfp express in S2 - (reply: 7)
219. 3' terminal post-transcriptional processing of miRNAs? - (reply: 2)
220. Double - Triple stable transfection - (reply: 4)
221. Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
222. qPCR for microRNAs - minimal primer length? (reply: 6)
223. mRNA induction after shRNA transfection - (reply: 3)
224. Loss of RNAi after changing culture media? - (reply: 2)
225. Dicer knockdown in mice - (reply: 1)
226. transfection agent -primary mouse macrophages, anybody? - (reply: 1)
227. cloning shRNA into pLentilox 3.7 - (reply: 3)
228. validated siRNA doesn't work - (reply: 6)
229. protein-protein interaction-plz help - (reply: 12)
230. Trouble cloning miRNAs - (reply: 5)
231. RNAi - vector construction (reply: 3)
232. polyA tail as 3' adaptor to clone miRNA - (reply: 1)
233. need protocol for precipitation of 13 mer mRNA - (reply: 3)
234. Has any one successfully performed miRNA cloning? - miRNA cloning troubleshooting (reply: 5)
235. sirna half life - time of effect (reply: 2)
236. Uridines in linker design for miRNA cloning. - Why uridine is often used in the 3ยด-Linkers. (reply: 2)
237. RNAase-free saline - (reply: 1)
238. shRNA control - (reply: 3)
239. Biotin ou HRP - (reply: 1)
240. transfection of chick cell - (reply: 3)