Protocol Online logo
Top : Forum Archives: : Indexed by Topic
Precipitaion Related Discussions
  1. dialysed sample white precipitate after lyophilization? - dialysed sample white precipitate after lyophilization? (reply: 2)
  2. precipitation in DMEM - (reply: 3)
  3. Is incubation absolutely necessary for alcohol precipitation of RNA? - I'm doing HIV RNA isolation from plasma. (reply: 1)
  4. Does Isopropanol freeze? - DNA precipitation (reply: 4)
  5. milky ethanol + viscous "pellet" - DNA precipitation (reply: 6)
  6. PMSF precipitate? - (reply: 5)
  7. RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
  8. RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
  9. DNA precipitation EtOH with Na OAc or Ammonium OAc - (reply: 4)
  10. White precipitation in stacking gel - (reply: 10)
  11. precipitation of protein with acetone - (reply: 3)
  12. precipitation during TEV cleavage - (reply: 1)
  13. question on ethanol precipitation - (reply: 1)
  14. problem dissolving protein pellet after aceton precipitation - (reply: 3)
  15. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
  16. How much salt can/should be used for DNA precipitation? - Is there a lower or upper limit? (reply: 8)
  17. how to pulldown membrane-bound protein? - co-immune precipitation (reply: 1)
  18. PEG didn't precipitate right away....what is your experience or advice? - PEG precipitation (reply: 8)
  19. 1M NaCl doesn't dissolve...not sure why - PEG precipitation of phages (reply: 2)
  20. calcium chloride in a phosphate buffer - how to avoid calcium phosphate precipitates (reply: 3)
  21. The role of salt in DNA precipitation - (reply: 3)
  22. RNA extraction - precipitation - (reply: 3)
  23. A way to avoid insoluble precipitate with sodium acetate - (reply: 9)
  24. protein precipitation - (reply: 2)
  25. precipitation of protein (PI=9.5) but not DNA - I need protein free sample for DNA quantification (reply: 2)
  26. TCA precipitation - (reply: 3)
  27. protein precipitation after enterokinase cleavage - (reply: 1)
  28. Precipitate proteins but not 1kDa peptide? - (reply: 3)
  29. About red precipitate during passing the cell line - (reply: 2)
  30. How can I avoid protein precipitation? - (reply: 7)
  31. Cant see ppt after ethanol precipitation?! - (reply: 3)
  32. Reverse phase chromatography of acetone protein precipitate - (reply: 2)
  33. RNA precipitation with DNase treatment - (reply: 1)
  34. Endofree DNA precipitation accident-- can I salvage my dna? - (reply: 1)
  35. ammonium sulfate precipitation & urea - (reply: 9)
  36. Precipitate Iron in Water - science fair question (reply: 4)
  37. ChIP: SDS precipitates during sonication...any ideas? - (reply: 7)
  38. Tips on PEG precipitation of DNA - (reply: 5)
  39. mistake during alcohol precipitation - (reply: 6)
  40. precipitation of guanidine HCl-sample after adding 4X laemmli buffer - SDS-PAGE protein sample preparation (reply: 2)
  41. Protein precipitation. Overdrying proteins - (reply: 3)
  42. Acetone precipitation - (reply: 2)
  43. seeking TCA precipitation advice - (reply: 2)
  44. Difficulty resuspending DNA after precipitation - (reply: 7)
  45. Ugly smears in SDS-PAGE after MeOH precipitation - (reply: 2)
  46. why low spins recommended in RNA precipitation trizol protocol? - Gibco, later Invitrogen recommend only max 12000g - why? (reply: 2)
  47. LiCl riboprobe precipitation - (reply: 1)
  48. Ethanol precipitation of protein - (reply: 2)
  49. pectinase precipitation - how to precipitate (reply: 2)
  50. Use cold TCA for protein precipitation? - (reply: 3)
  51. Anyone know how can I precipitate PCR product in 96-well plate? - (reply: 2)
  52. Problem with TCA precipitation. - can't see pellet, what should i do..VERY URGENT..!!!!!&# (reply: 2)
  53. pectin production - an alternative for alcohol precipitation - (reply: 1)
  54. protein salt precipitation - (reply: 2)
  55. Problems with inconsitant protein recovery using ammonium sulphate (AS) precipit - protein precipitation/concentration (reply: 2)
  56. Licl precipitation: -80°C or -20°C; with/without ethanol; 1hour or overnightl - (reply: 1)
  57. DNA, RNA, protein acetone precipitation? - (reply: 2)
  58. Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
  59. Protein precipitation compatible with Sucrose gradients? - What precipitation protocol wil function with a high % of sucrose? (reply: 5)
  60. What is the most effective method of protein precipitation? - (reply: 2)
  61. HPLC acetone Precipitation SDS-PAGE not Working - (reply: 1)
  62. Can alcohol precipitation give 100% recovery of DNA? - (reply: 3)
  63. TCA radiacive protein precipitation without lysis - (reply: 1)
  64. DNA Precipitaion - (reply: 3)
  65. salt precipitation (for protein/collagen) - (reply: 1)
  66. Protein precipitation during dialysis - (reply: 7)
  67. IgG purification - amonium precipitation method (reply: 5)
  68. Protein precipitation with PEG 4000 - (reply: 4)
  69. Ammonium acetate precipitation of DNA - Help is neede for ammonium acetate precipitation of DNA (reply: 6)
  70. Help with DNA precipitation - (reply: 1)
  71. TCA precipitation, what is the best solution to dissolve followed by Mass spectr - (reply: 1)
  72. Precipitation of peptide from a large volume - (reply: 14)
  73. Need to increase protein precipitation yield w/ TCA precipitation protocol - (reply: 3)
  74. EDTA first or iron ?! - precipitation (reply: 16)
  75. need protocol for precipitation of 13 mer mRNA - (reply: 3)
  76. About the ammonium sulphate precipitation? - prootein purification (reply: 10)
  77. sodium acetate for DNA precipitation - (reply: 1)
  78. SDS Precipitation with 1% Lysis Buffer after 10' on ice - (reply: 1)
  79. how can I dialyze protein through its PI and avoid precipitation? - (reply: 1)
  80. Acetone Precipitation - (reply: 2)
  81. precipitation of phospho-protein - (reply: 1)
  82. why my freshly prepared 30% Acrylamide solution has precipitate? - (reply: 10)
  83. White precipitate after unfreezing from -20 degrees centigrade - (reply: 4)
  84. sodium acetate - the function of soduim acetate in precipitation of DNA/RNA (reply: 2)
  85. calcium chloride precipitation problem - (reply: 1)
  86. Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
  87. concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
  88. DNA preparation for Chromatin Immuno Precipitation - (reply: 2)
  89. My antibody is forming a precipitate! - Why? (reply: 7)
  90. antibody concentration - antibody precipitation using ammonium sulfate (reply: 2)
  91. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
  92. Ethanol precipitation of protein sample - What is the protocol? (reply: 4)
  93. 3X flag peptide precipitate out - (reply: 1)
  94. ethanol precipitation before gel purification... - (reply: 5)
  95. protein precipitation upon dialysis - (reply: 5)
  96. precipitate formation - transfection with calcium phosphate precipitaion (reply: 2)
  97. precipitation of nuclear proteins during GST pull down assay - (reply: 1)
  98. Resuspended DNA in false buffer! - How to precipitate? (reply: 5)
  99. can I just ethanol precipitate Klenow? - without deactivating it? (reply: 4)
  100. SDS-PAGE precipitation& denaturation - (reply: 3)
  101. Problem with minimal isopropanol precipitation - (reply: 2)
  102. SDS-PAGE protein aggregation and precipitation - (reply: 1)
  103. DNA precipitation and not protein - specific.. need it now!! (reply: 2)
  104. Problems with Plasmid purification - no real precipitation of genomic DNA (reply: 8)
  105. Ethanol precipitation vs Clean up kit? - which will yield more (reply: 16)
  106. Plasmid DNA problem - PEG precipitation (reply: 3)
  107. Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
  108. Protocol for Re-ethanol precipitation of already purified DNA - (reply: 3)
  109. Explanation of DNA Precipitation - (reply: 4)
  110. Ammonium Sulfate precipitation...issues - (reply: 1)
  111. can protein A agarose precipitate anti goat IgG? - (reply: 2)
  112. miniprep: no precipitate after PEG treatment. - (reply: 4)
  113. asking about the ammonium sulphate precipitation - (reply: 4)
  114. protein precipitation - (reply: 4)
  115. Protein TCA Precipitation Resolubilization Question - (reply: 3)
  116. %recovery of 26mer-oligo-T purification by ethanol precipitation is very low - other oligonucleotides were about 80-95% (reply: 3)
  117. Problem with protein refolding - protein precipitate all the time !!! why (reply: 2)
  118. Can DNA ethanol precipitation remove restriction enzyme? - (reply: 7)
  119. TCA/Acetone precipitation - (reply: 3)
  120. Temperature for EtOH Precipitation? - (reply: 6)
  121. adding glycogen to isoprop for precipitation? - (reply: 15)
  122. Protein Precipitation (wessel and Fluege method) - 2 questions (reply: 7)
  123. Protein precipitates - (reply: 7)
  124. what buffer will prevent the precipitation of protein at -20? - (reply: 5)
  125. EtOH precipitation of RNA problems - (reply: 5)
  126. Ethanol precipitation following sucrose gradient - problem - (reply: 7)
  127. How does protein precipitate? - (reply: 1)
  128. pattern of DNA precipitation - (reply: 3)
  129. protein precipitation during concentration - (reply: 5)
  130. Precipitate formation of BSA + DTT - (reply: 9)
  131. can't stop the precipitate! - adding cacl2 to pk buffer (reply: 6)
  132. sodium acetate precipitation times - (reply: 7)
  133. Best way to precipitate/concentrate genomic DNA? - (reply: 4)
  134. PEG precipitation of phage - (reply: 3)
  135. What’s the difference between ethanol and isopropanol precipitation? - (reply: 3)
  136. Appropriate salt for DNA precipitation - (reply: 3)
  137. Protein disappeared after TCA precipitation - (reply: 7)
  138. Iso-propanal precipitation - (reply: 9)
  139. how to deal with the precipitation of calciumnitrate and dipotassiumhydrogenphos - (reply: 1)
  140. precipitate coming out of media! - (reply: 12)
  141. precipitation of Sodiummolibdatedihydrate, this is so weird? - (reply: 1)
  142. RNA precipitation - (reply: 5)
  143. Ethanol precipitation problem - (reply: 7)
  144. Is it obligatory to precipitate DNA from ligation before transformed into E.coli - (reply: 7)
  145. protein precipitation / RNA binding factors - (reply: 3)
  146. Has anyone experienced FCS precipitation after heat inactivation? - (reply: 1)
  147. cDNA precipitate & DNA ladders - (reply: 4)
  148. Protein precipitation using Methanol/Chloroform - (reply: 1)
  149. help with oligonucleotide precipitation assay - (reply: 1)
  150. salt precipitation of diverse collagen types - (reply: 1)
  151. protein precipitation for mouse liver - (reply: 1)
  152. protein precipitation during gel loading - (reply: 3)
  153. Adding "volumes" of ethanol to precipitate nucleic acids - (reply: 7)
  154. LiCl precipitation time - (reply: 1)
  155. the centrifuge speed to precipitate the 30bp oligo - (reply: 5)
  156. Nonspecific precipitation in ChIP - (reply: 4)
  157. Buffer for amonium sulphate precipitation - (reply: 1)
  158. How do YOU precipitate your RNA? - (reply: 2)
  159. Precipitation of 10X TBE - (reply: 18)
  160. Gel Filtration/Protein Precipitation - (reply: 1)
  161. For all the DNA gurus....help with "invisible" pellet - Problematic EtOH precipitation (reply: 5)
  162. How does glycogen in DNA precipitation work? - (Ethanol, sodium acetate and glycogen precipitation) (reply: 4)
  163. TCA/DOC precipitation - (reply: 1)
  164. Phage precipitation techniques? - (reply: 1)
  165. DNA precipitation directly from serum - Is it possible direct precipitation of DNA from serum (reply: 1)
  166. White precipitation in PCR - ever seen this? (reply: 4)
  167. Role of Sodium Acetate in DNA precipitation - (reply: 3)
  168. PCR product precipitation - (reply: 4)
  169. purify Ab precipitate after neutralize to pH7 - (reply: 2)
  170. DNA precipitation with ethanol - (reply: 1)
  171. Protein precipitation problems - (reply: 3)
  172. HELP: ph precipitation - (reply: 2)
  173. pH and precipitation - (reply: 2)
  174. How to avoid getting a precipitate with Giemsa staining??? - (reply: 2)
  175. isopropanol precipitate lysis? - (reply: 1)
  176. DNA extraction - details about ethanol precipitation - (reply: 12)
  177. Ammonium acetate precipitation of NaOH treated DNA - (reply: 6)
  178. How to prepare 4M ammonium sulphate solution? - Ammonium sulphate precipitation (reply: 5)
  179. why did precipitate formed after antibody freez/thaw ? - (reply: 2)
  180. TCA precipitation - (reply: 6)
  181. Anyone else get the black dot in their nucleic acid precipitate? - (reply: 4)
  182. RNA precipitation - How long should I precipitate my RNA? (reply: 2)
  183. How ethanol precipitation works? - (reply: 2)
  184. agglutination and precipitation - when we can use agglutination and precipitation fo (reply: 1)
  185. question about DNA precipitation - (reply: 2)
  186. Is this a bacterial Contamination? - White precipitate on surface of medium (reply: 1)
  187. DNA extraction - white precipitate? - DNA extraction contamination (reply: 5)
  188. help-ammonium sulfate precipitation - (reply: 1)
  189. Chloroform and DNA precipitation - (reply: 2)
  190. DNA pellet (?) floats in CTAB precipitation - (reply: 2)
  191. Ethanol precipitation - do you mix after adding salt? - (reply: 4)
  192. having hard time to re solubilize after protein aceton ppt - protein precipitation (reply: 6)
  193. PEG precipitation, what kinds of PEG I should use? - virology (reply: 2)
  194. Tips on ethanol precipitation of nucleic acids and wash - (reply: 48)
  195. protein precipitation after IMAC - protein purification (reply: 1)
  196. TCA protein precipitation - (reply: 2)
  197. Isopropanol precipitation - best time and temperature? - (reply: 6)
  198. precipitation with ammonium sulfate - (reply: 1)
  199. How resuspend protein after (NH4)2SO4 precipitation? - (reply: 2)
  200. Precipitation with TCA and Na deoxycholate - what is the role of deoxycholate? (reply: 4)
  201. Ethanol vs Isopropanol for DNA precipitation - (reply: 37)
  202. What's the function of NaAc in DNA precipitation(using Eth)? - (reply: 5)
  203. TCA precipitation of proteins - (reply: 15)
  204. Precipitation of DNA from CsCl samples - (reply: 1)
  205. the precipitate on DEPC-H2O bottle - (reply: 1)
  206. ammonium sulfate precipitation - (reply: 1)
  207. protein precipitation - protein precipitation (reply: 5)
  208. Separating protein from sucrose by precipitation? - help! (reply: 1)
  209. Precipitation of Native Protein - IMAC of Native Protein (reply: 1)
  210. protein precipitates after dialysis - Protein purification (reply: 4)
  211. prevention of protein precipitation during dialysis - (reply: 3)
  212. DNA precipitation by EtOH &centrifuge - (reply: 4)
  213. TCA precipitation in presence ofurea - (reply: 1)
  214. Ammonium sulfate precipitaion problem - General Methods (reply: 1)
  215. how to precipitate the nucleicacid with out proteins - (reply: 2)
  216. RNA precipitation - (reply: 7)