White precipitation in PCR - ever seen this? (Oct/28/2005 )
Hi!
Recently we observed a white precipitation in our tubes after PCR. It's sort of slimy and This can be observed even in the negative control (i.e. without any DNA)!
Have ever you seen something like this? We can't explain it! The only new reaagent is the primers. Can it has something to do with it? Or how can you explain this?
*confused*
Hello
We had find this sometimes when using BSA in the pcr-mix. You use BSA or something like this on your mix??
We run later on the samples on DGGE gels and find no problem.
Yours
CAr
We had find this sometimes when using BSA in the pcr-mix. You use BSA or something like this on your mix??
We run later on the samples on DGGE gels and find no problem.
Yours
CAr
Yes, we use BSA in the reaction. Hm... sounds reasonable. Thanks for the explanation!
But now I have another problem... We've found that this precipitation only occurs when one of our lab personel makes the PCR! When I run the PCR, nothing is percipitated. Is there something in the handeling that can cause the BSA to precipitate?
*now less confused*
Hmmmm.....powder from gloves?
Hmmm, don't know. I wouldn't let them handle my PCR, RNA, or cell culture, though, that's for sure!
On a side note, I would not use BSA in PCR because of its propensity for decreasing specificity.
-Matt