Acetone Precipitation - (Apr/11/2007 )

Just a quick question:

does acetone precipitation alter the phosphorylation state of a protein??

cheers

-Jimmy_september-

no, it shouldn't.

-mdfenko-

QUOTE (Jimmy_september @ Apr 11 2007, 12:09 PM)

Just a quick question:

does acetone precipitation alter the phosphorylation state of a protein??

cheers

it does not forward hydrolysation; acetone is used in the classical Roskoski protocol for in vitro phosphorylation to dry the phosphocellulose paper

-The Bearer-