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Oligonucleotide Related Discussions
  1. Detection of annealed oligos - (reply: 3)
  2. oligo modification - to prevent the binding of SSB protein (reply: 1)
  3. ligation and transformation problems - (wrong) oligos (reply: 4)
  4. How much oligo dT cDNA to use in PCR - (reply: 3)
  5. Help with non-radioactive EMSA! - unlabeled oligo causes bands to appear instead of disappear!!! (reply: 4)
  6. Oligo dT primer - oligo dT primer with non-T priming 3´ or with 5´ (reply: 3)
  7. Solubility of Oligonucleotides? - (reply: 2)
  8. annealing complementary oligos prior to biotin labeling - (reply: 1)
  9. calculating nunber of copies of synthetic RNA oligo - (reply: 1)
  10. emsa oligo annealing - biotin labelled oligos (reply: 5)
  11. emsa competition oligos - how to make up competition oligos (reply: 3)
  12. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  13. oligos phosphorylation with PNK - (reply: 6)
  14. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  15. oligos phosphorylation with pnk - (reply: 4)
  16. Melting Temperature of Oligos - (reply: 1)
  17. SPECIFICITY of extra bases added beside RE site in OLIGO for PCRing insert - (reply: 2)
  18. antisense oligo - (reply: 1)
  19. 2'-O-methyl oligoribonucleotides as miRNA inhibitor - how many nucleotides in the oligo need to be modified (reply: 1)
  20. site directed mutagenesis using synthesized oligos - (reply: 1)
  21. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
  22. mRNA purification, stratagene - principles of mRNA oligo(dT) magnetic particles (reply: 3)
  23. RNAi or Antisense oligos for inhibition in vitro and in vivo - (reply: 1)
  24. short oligos ligation and transformation - (reply: 3)
  25. Site-directed mutagenesis problem - Mutations under oligo (reply: 2)
  26. Experience with Ultramers from IDT? - Long oligos (reply: 1)
  27. Salt for annealing oligos - (reply: 2)
  28. Crosslinking of oligos to membrane required for EMSA? - Is it necessary to crosslink the oligos to a positively charged nylon (reply: 2)
  29. annealing a self complementary, symmetrical oligo - (reply: 2)
  30. QPCR Oligo Database of Pathogens - (reply: 1)
  31. Double-stranded oligo ligation - (reply: 13)
  32. annealing oligos for EMSA - (reply: 1)
  33. GC rich oligo - (reply: 2)
  34. Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
  35. chimeric RNA-DNA oligo probes for northern - sensitivity in miRNA northern detection (reply: 1)
  36. Annealing and Ligating Oligos - (reply: 1)
  37. Annealing and cloning of four single stranded oligos - (reply: 1)
  38. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  39. 3' bioninylated oligos for EMSA - (reply: 2)
  40. how to ligate random oligos to vector to construct a library? - (reply: 6)
  41. Synthetic DNA: anneal from Oligos or not? - Assemble from oligos or buy it outright? (reply: 20)
  42. Purification after oligos phosphorylation? - (reply: 1)
  43. annealing oligo to a cut vector - (reply: 3)
  44. oligo annealing - (reply: 3)
  45. Cloning Oligos - (reply: 2)
  46. Standard oligos T7 and T3 - (reply: 3)
  47. What is antisense phosphorothioate oligonucleotides? - (reply: 2)
  48. miRNA inhibitors - 2'-O-methyl versus Ambion antisense oligos (reply: 4)
  49. Oligo concentration - (reply: 3)
  50. differences between oligo and random primers - (reply: 2)
  51. Random Primers vs Oligo(dT) primers? - (reply: 3)
  52. %recovery of 26mer-oligo-T purification by ethanol precipitation is very low - other oligonucleotides were about 80-95% (reply: 3)
  53. Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
  54. oligo(dT) plus random primers in RT - (reply: 4)
  55. storage of oligo dT and primer - storage condition (reply: 2)
  56. Suggestions for oligo modifications - (reply: 6)
  57. size of probes for northern blot and other questions - end labelling of 20 mer oligonucleotides (reply: 3)
  58. RNA Oligos VS DNA oligos - Dumb Question but need a straight answer (reply: 4)
  59. Oligo design program? - (reply: 1)
  60. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
  61. Annealing and extending oligos - annealing and extention of single-stranded oligo to double stranded (reply: 6)
  62. help with oligonucleotide precipitation assay - (reply: 1)
  63. which method is more efficient for oligo end-labeling? - (reply: 6)
  64. Do you synthsize your own oligo(dT) primer? - (reply: 2)
  65. RNA storing/RT PCR using oligo dT - (reply: 5)
  66. Size exclusion spin columns for oligo purification ... - (reply: 1)
  67. the centrifuge speed to precipitate the 30bp oligo - (reply: 5)
  68. biotinylated oligos - (reply: 6)
  69. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  70. Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
  71. Spin column for purification of oligonucleotides - (reply: 2)
  72. Annealing of oligos - (reply: 3)
  73. Antisense Oligo nucleotide designing - (reply: 2)
  74. Molar concentration help - prepare an oligo 200 pmol/ul (reply: 6)
  75. Oligo(dT)18 vs. Oligo(dT) 20? - (reply: 2)
  76. oligo concentration calculation - (reply: 5)
  77. Labelling oligo with biotin during synthesis for EMSA - (reply: 2)
  78. Annealing and labeling oligo probe for gel-shift assay - (reply: 1)
  79. SuperArray: cDNA versus oligo - (reply: 1)
  80. ligating small oligos - how to ligate small oligos into vector (reply: 2)
  81. oligonucleotide Adaptor A - (reply: 1)
  82. ligation of short oligos - (reply: 3)
  83. Re-suspending oligos in TE, Tris or water - (reply: 4)
  84. phosphorylated oligos and vector - (reply: 2)
  85. designing Oligos for Northern - (reply: 1)
  86. Long Oligonucleotide Arrays for Human - (reply: 1)
  87. Annealing short oligos - How to anneal short oligos? (reply: 7)
  88. Oligo purification - (reply: 5)
  89. transfection with AS oligos ~ control? - (reply: 7)
  90. Ligation of two oligos into a vector - (reply: 4)
  91. Oligonucleotide microarray - (reply: 1)
  92. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
  93. difficulty in cloning oligos into vector - cloning difficulties (reply: 1)
  94. oligo annealing - how many oligos for annealing (reply: 1)
  95. Tool for searching optimal oligonucleotide, ISH? - (reply: 2)
  96. DNA Methylation in vitro - How to carry out DNA methylation on a given oligo (reply: 3)
  97. Making dsDNA from an ssDNA oligo - (reply: 2)
  98. poor ligation of plasmid/oligo construct - (reply: 8)
  99. Working with DNA, RNA and oligonucleotides - (reply: 6)
  100. dsOligos annealing buffer - (reply: 2)
  101. Help! Three way ligation including blunt-end oligo - (reply: 2)
  102. Removing Oligonucleotides - (reply: 2)
  103. How to prepare double-stranded oligonucleotides? - from synthesized oligos (reply: 5)
  104. Oligo design strategy - cloning in pQE 30 (QIAGEN°) (reply: 4)
  105. designing antisense oligo's... - (reply: 5)
  106. Oligo calculator - (reply: 1)
  107. oligo primers - info (reply: 2)
  108. Re: oligo probe - (reply: 2)
  109. antisense oligos - (reply: 1)
  110. oligo cloning - (reply: 2)
  111. oligo hybridization and ligation - hybridization and ligation (reply: 1)
  112. Antisense oligodeoxynucleotides - (reply: 1)
  113. storing oligos - (reply: 3)