designing Oligos for Northern - (Aug/08/2005 )
Hi Friends
I want to design Oligos for Northern Hybridisation. I would be glad to know if there are any programs available online, (like the ones for Primer designing like Primer3, InsilicoPCR from UCSC etc...) I also want to check the hybridized (to be) product Insilico, if possible....( something like Reverse blast???)...
Please let me know if any one of you can help me out
thanks in advance
Chenna
-galiveti-
QUOTE (galiveti @ Aug 8 2005, 10:18 AM)
Hi Friends
I want to design Oligos for Northern Hybridisation. I would be glad to know if there are any programs available online, (like the ones for Primer designing like Primer3, InsilicoPCR from UCSC etc...) I also want to check the hybridized (to be) product Insilico, if possible....( something like Reverse blast???)...
Please let me know if any one of you can help me out
thanks in advance
Chenna
I want to design Oligos for Northern Hybridisation. I would be glad to know if there are any programs available online, (like the ones for Primer designing like Primer3, InsilicoPCR from UCSC etc...) I also want to check the hybridized (to be) product Insilico, if possible....( something like Reverse blast???)...
Please let me know if any one of you can help me out
thanks in advance
Chenna
How large do you plan on having your probe? Depending on the size, ordering oligo's may work. I've always done run-off transcription reactions to generate my probes though.
-Hank
-haringsh-