annealing a self complementary, symmetrical oligo - (Jun/13/2007 )
I am trying to anneal a synthetic self complementary, symmetrical oligo that keeps forming a hairpin. Does anyone have any suggestions to try that can make this form a double strand?
Thanks
Billy
-buddwt-
well... i anneal previously 5-6 oligos that encode for hairpin structure after their transcription, and it's just like standard annealing.
heat in STE buffer and allow to cool. Then check on agarose gel
-fred_33-
Just normal annealing protocol will work. we use it for shRNA stuff.
-scolix-