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annealing a self complementary, symmetrical oligo - (Jun/13/2007 )

I am trying to anneal a synthetic self complementary, symmetrical oligo that keeps forming a hairpin. Does anyone have any suggestions to try that can make this form a double strand?
Thanks
Billy

-buddwt-

well... i anneal previously 5-6 oligos that encode for hairpin structure after their transcription, and it's just like standard annealing.
heat in STE buffer and allow to cool. Then check on agarose gel

-fred_33-

Just normal annealing protocol will work. we use it for shRNA stuff.

-scolix-