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211. Proper ratio of stacking and separating gel - (reply: 3)
212. SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 3)
213. band size with flag tagged proteins? - (reply: 3)
214. benefits of urea in SDS PAGE gel - (reply: 9)
215. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)
216. Reuse WB - (reply: 3)
217. Stripping for Western blots - What is the common procedure for stripping? (reply: 4)
218. peroblems with chemiluminescent detection on films - (reply: 2)
219. protein elution from SDS PAGE - any idea how to elute protein from SDS PAGE (reply: 1)
220. Some questions... - (reply: 4)
221. western blot -- running the gel - (reply: 6)
222. Cell lysate at RT - (reply: 4)
223. Circular cassette imprint on membrane - (reply: 2)
224. what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
225. protein denaturation - (reply: 3)
226. bands sizes with sectreted protein - (reply: 1)
227. When dot blotting - (reply: 2)
228. Help! low molecule weight peptides electrophoresis! - (reply: 2)
229. Blots with glycopeptides - (reply: 2)
230. silly question about loading control - (reply: 4)
231. western blot current setting.. for 1 or 2 blots together? - (reply: 1)
232. Yellow Laemmli Sample Buffer - (reply: 15)
233. cell lysate PAGE & blotting problem? - (reply: 1)
234. Native PAGE problem - Protein doesn't enter in resolving gel (reply: 1)
235. Wester blot non specific bands caused by secondary - (reply: 3)
236. HA antibody - (reply: 1)
237. selection of buffer for dialysis? - (reply: 1)
238. Native gels for proteasome activity assay - (reply: 3)
239. SDS-PAGE Mercaptoethanol or - (reply: 3)
240. BSA vs. Nonfat dry milk for serine/threonine phosphorylation sites - which is better for quality blots? (reply: 1)
212. SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 3)
213. band size with flag tagged proteins? - (reply: 3)
214. benefits of urea in SDS PAGE gel - (reply: 9)
215. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)
216. Reuse WB - (reply: 3)
217. Stripping for Western blots - What is the common procedure for stripping? (reply: 4)
218. peroblems with chemiluminescent detection on films - (reply: 2)
219. protein elution from SDS PAGE - any idea how to elute protein from SDS PAGE (reply: 1)
220. Some questions... - (reply: 4)
221. western blot -- running the gel - (reply: 6)
222. Cell lysate at RT - (reply: 4)
223. Circular cassette imprint on membrane - (reply: 2)
224. what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
225. protein denaturation - (reply: 3)
226. bands sizes with sectreted protein - (reply: 1)
227. When dot blotting - (reply: 2)
228. Help! low molecule weight peptides electrophoresis! - (reply: 2)
229. Blots with glycopeptides - (reply: 2)
230. silly question about loading control - (reply: 4)
231. western blot current setting.. for 1 or 2 blots together? - (reply: 1)
232. Yellow Laemmli Sample Buffer - (reply: 15)
233. cell lysate PAGE & blotting problem? - (reply: 1)
234. Native PAGE problem - Protein doesn't enter in resolving gel (reply: 1)
235. Wester blot non specific bands caused by secondary - (reply: 3)
236. HA antibody - (reply: 1)
237. selection of buffer for dialysis? - (reply: 1)
238. Native gels for proteasome activity assay - (reply: 3)
239. SDS-PAGE Mercaptoethanol or - (reply: 3)
240. BSA vs. Nonfat dry milk for serine/threonine phosphorylation sites - which is better for quality blots? (reply: 1)