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271. Just a smear of proteins after ponceau red - Western Blot (reply: 4)
272. Storing protein extracted from tissues for Western blot - (reply: 9)
273. big dye tails on SDS PAGE, picture attached - (reply: 4)
274. How to quantify protein which has sds buffer already - (reply: 1)
275. different western results when using acetone prep vs crude protein - (reply: 3)
276. Gap43 protein western Blot - (reply: 7)
277. help, :-( Actin completely blank on western blot - (reply: 6)
278. pipetting technique for western blot sampling - (reply: 3)
279. Problem with protein extraction - (reply: 2)
280. SDS Gel - My protein bands go to the bottom of gel (reply: 9)
281. SDS PAGE Inclusion bodies - (reply: 2)
282. 2 - DE Gel - Protein Transfer - (reply: 2)
283. 10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
284. How much of antibody to add? - (reply: 3)
285. Does sonication of cell lysate.... - (reply: 2)
286. signal is weak or fading - (reply: 8)
287. native Vs SDS PAGE - (reply: 5)
288. Dimer bands under reducing conditions - (reply: 1)
289. comassie G90 - staining (reply: 1)
290. Tris tricine SDS page blotting - (reply: 4)
291. Separation of similar weight protiens - (reply: 6)
292. alpha tubulin band the wrong size! - (reply: 2)
293. Molecular weight of haemoglobin subunits - (reply: 2)
294. how to boil samples before load to SDS-PAGE? - (reply: 11)
295. Caspase-1 Western spontaneous activation - Need help (reply: 1)
296. protein an its phospho-protein in the same membrane - (reply: 3)
297. IGF1 westerns - Do you have a nice protocol? (reply: 8)
298. How can I load equal amounts of protein into each well during SDS-PAGE? - SDS-PAGE, WB, BCA assay, Actin (reply: 7)
299. problem with western blot - (reply: 4)
300. tris tricine SDS PAGE - how to visualize under 2kDa (reply: 2)
272. Storing protein extracted from tissues for Western blot - (reply: 9)
273. big dye tails on SDS PAGE, picture attached - (reply: 4)
274. How to quantify protein which has sds buffer already - (reply: 1)
275. different western results when using acetone prep vs crude protein - (reply: 3)
276. Gap43 protein western Blot - (reply: 7)
277. help, :-( Actin completely blank on western blot - (reply: 6)
278. pipetting technique for western blot sampling - (reply: 3)
279. Problem with protein extraction - (reply: 2)
280. SDS Gel - My protein bands go to the bottom of gel (reply: 9)
281. SDS PAGE Inclusion bodies - (reply: 2)
282. 2 - DE Gel - Protein Transfer - (reply: 2)
283. 10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
284. How much of antibody to add? - (reply: 3)
285. Does sonication of cell lysate.... - (reply: 2)
286. signal is weak or fading - (reply: 8)
287. native Vs SDS PAGE - (reply: 5)
288. Dimer bands under reducing conditions - (reply: 1)
289. comassie G90 - staining (reply: 1)
290. Tris tricine SDS page blotting - (reply: 4)
291. Separation of similar weight protiens - (reply: 6)
292. alpha tubulin band the wrong size! - (reply: 2)
293. Molecular weight of haemoglobin subunits - (reply: 2)
294. how to boil samples before load to SDS-PAGE? - (reply: 11)
295. Caspase-1 Western spontaneous activation - Need help (reply: 1)
296. protein an its phospho-protein in the same membrane - (reply: 3)
297. IGF1 westerns - Do you have a nice protocol? (reply: 8)
298. How can I load equal amounts of protein into each well during SDS-PAGE? - SDS-PAGE, WB, BCA assay, Actin (reply: 7)
299. problem with western blot - (reply: 4)
300. tris tricine SDS PAGE - how to visualize under 2kDa (reply: 2)