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31. optimal conditions for wet transfer in western blotting - (reply: 3)
32. Laemmli buffer recipe - (reply: 3)
33. separation of loading buffer - (reply: 1)
34. zymogram of pectinase - activity staining of pectinase (reply: 2)
35. Immunoprecipitation - Immunoprecipitation protocol to obtain native immunoprecipitated prots (reply: 3)
36. western blot, I have a signal without primary antibody - (reply: 6)
37. transfer of high molecular weight proteins - (reply: 11)
38. Quench HRP with sodium azide - (reply: 3)
39. recipe for LDS based sample buffer - (reply: 1)
40. bubbles and overheating of western blot - (reply: 2)
41. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
42. band extraction from sds page - (reply: 1)
43. Same samples but different results! - (reply: 3)
44. phosphoSer lower on films - (reply: 2)
45. Western blot control - Suggestions? (reply: 3)
46. Loading control for tears WB - GAPDH? (reply: 4)
47. western blot from heart lysates - membrane extractions too (reply: 2)
48. Problems with blot - (reply: 2)
49. protein concentration problem - (reply: 2)
50. Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
51. White precipitation in stacking gel - (reply: 10)
52. cell lysis buffer to use directly on the dish - (reply: 1)
53. Loading control - (reply: 5)
54. Frowning and Smiling - problems with SDS-PAGE (reply: 5)
55. Fixing Current vs Voltage in Transfer - (reply: 1)
56. optimal timepoint to collect secreted protein - (reply: 1)
57. SDS buffers - (reply: 1)
58. SDS buffers - (reply: 1)
59. SDS buffer - (reply: 1)
60. Boiling a Western Blot? - Does it work? (reply: 3)
32. Laemmli buffer recipe - (reply: 3)
33. separation of loading buffer - (reply: 1)
34. zymogram of pectinase - activity staining of pectinase (reply: 2)
35. Immunoprecipitation - Immunoprecipitation protocol to obtain native immunoprecipitated prots (reply: 3)
36. western blot, I have a signal without primary antibody - (reply: 6)
37. transfer of high molecular weight proteins - (reply: 11)
38. Quench HRP with sodium azide - (reply: 3)
39. recipe for LDS based sample buffer - (reply: 1)
40. bubbles and overheating of western blot - (reply: 2)
41. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
42. band extraction from sds page - (reply: 1)
43. Same samples but different results! - (reply: 3)
44. phosphoSer lower on films - (reply: 2)
45. Western blot control - Suggestions? (reply: 3)
46. Loading control for tears WB - GAPDH? (reply: 4)
47. western blot from heart lysates - membrane extractions too (reply: 2)
48. Problems with blot - (reply: 2)
49. protein concentration problem - (reply: 2)
50. Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
51. White precipitation in stacking gel - (reply: 10)
52. cell lysis buffer to use directly on the dish - (reply: 1)
53. Loading control - (reply: 5)
54. Frowning and Smiling - problems with SDS-PAGE (reply: 5)
55. Fixing Current vs Voltage in Transfer - (reply: 1)
56. optimal timepoint to collect secreted protein - (reply: 1)
57. SDS buffers - (reply: 1)
58. SDS buffers - (reply: 1)
59. SDS buffer - (reply: 1)
60. Boiling a Western Blot? - Does it work? (reply: 3)