peroblems with chemiluminescent detection on films - (Apr/17/2008 )

Hello,
I have problem with chemiluminescent detection on X-ray films. I can't get signal on the film but I can see it on the membrane. We use nitrocelulose membrane for transfer, AGFA blue films and universal developer and fixer, also we use Roche Lumi-Light substrate and I never had any problems with detection till now. I don' know what to do, I tried differnt exposition time from 2 min till over night. Also I tried longer incubation in luminol and it doesn't help.
Has anyone got the clue what could be wrong?

Thanks

Hi there,
I had the same problem with those AGFA films. With some proteins they seem to be really insensitive.
Do you have a positive control on your membrane so you can exclude that that the problem might be the film?