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1. New blot problems - (reply: 4)
2. Drying Neupage gel - how to achieveing drying and prevent cracking (reply: 1)
3. Protein Loading and Western Blot - Western Blot (reply: 5)
4. Unidentified problem with low concentrated samples - Variable limit of detection of my western (reply: 9)
5. Western Blot: PSMAD 1/5/8 low signal! HELPPPPP! - HELP! (reply: 1)
6. mitochondrial extraction immunoblot - what can be used for calibrating the amount of protein apart from VDAC (reply: 1)
7. pI and Transfer Buffer - (reply: 1)
8. Strange aspect of bands in Western Blot - (reply: 2)
9. Trouble-shooting Tris-Tricine SDS-PAGE - (reply: 5)
10. Concentration of EDTA in lysis buffer - (reply: 1)
11. Why low fold change (treatment vs. control)? - (reply: 5)
12. Samples not on a straight line caused by gel dryness? - (reply: 4)
13. How can i dual with wrong size band? - (reply: 1)
14. Size of protein - (reply: 4)
15. Actin Antibody short-lived? - (reply: 2)
16. double bands - (reply: 3)
17. protein concentration - (reply: 4)
18. 3X HA tag - (reply: 1)
19. mysterious thing! - (reply: 2)
20. How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
21. troubles with small proteins (less than 20) on SDS page - (reply: 5)
22. stripping buffer! - didn't strip after 30min (reply: 3)
23. protein spreading in gel - (reply: 1)
24. PVDF and Nitrocellulose have different current V/mAmp! - why am I seeing less transfer while using PVDF? (reply: 3)
25. Tris Hcl running gel buffer problems - (reply: 6)
26. transfer problem - blue nitrocellulose paper (reply: 3)
27. sds-gel problem - (reply: 2)
28. SDS-PAGE problem - no bands around 20KDa (reply: 3)
29. Marker for Sample from Nuclear Extraction - (reply: 2)
30. Gels waiting for 90 min before transfer? - (reply: 6)
2. Drying Neupage gel - how to achieveing drying and prevent cracking (reply: 1)
3. Protein Loading and Western Blot - Western Blot (reply: 5)
4. Unidentified problem with low concentrated samples - Variable limit of detection of my western (reply: 9)
5. Western Blot: PSMAD 1/5/8 low signal! HELPPPPP! - HELP! (reply: 1)
6. mitochondrial extraction immunoblot - what can be used for calibrating the amount of protein apart from VDAC (reply: 1)
7. pI and Transfer Buffer - (reply: 1)
8. Strange aspect of bands in Western Blot - (reply: 2)
9. Trouble-shooting Tris-Tricine SDS-PAGE - (reply: 5)
10. Concentration of EDTA in lysis buffer - (reply: 1)
11. Why low fold change (treatment vs. control)? - (reply: 5)
12. Samples not on a straight line caused by gel dryness? - (reply: 4)
13. How can i dual with wrong size band? - (reply: 1)
14. Size of protein - (reply: 4)
15. Actin Antibody short-lived? - (reply: 2)
16. double bands - (reply: 3)
17. protein concentration - (reply: 4)
18. 3X HA tag - (reply: 1)
19. mysterious thing! - (reply: 2)
20. How many O.D. cells to load onto SDS-PAGE? - (reply: 2)
21. troubles with small proteins (less than 20) on SDS page - (reply: 5)
22. stripping buffer! - didn't strip after 30min (reply: 3)
23. protein spreading in gel - (reply: 1)
24. PVDF and Nitrocellulose have different current V/mAmp! - why am I seeing less transfer while using PVDF? (reply: 3)
25. Tris Hcl running gel buffer problems - (reply: 6)
26. transfer problem - blue nitrocellulose paper (reply: 3)
27. sds-gel problem - (reply: 2)
28. SDS-PAGE problem - no bands around 20KDa (reply: 3)
29. Marker for Sample from Nuclear Extraction - (reply: 2)
30. Gels waiting for 90 min before transfer? - (reply: 6)