|
151. Yeast Western - (reply: 1)
152. Which quantity of purified protein in WB? - (reply: 2)
153. Sample Buffer - (reply: 1)
154. Which quantity of purified protein in WB? - (reply: 1)
155. 10% acrylamide/bisacrylamide, or 15% ? - SDS PAGE gel making (reply: 2)
156. Why my target protein did not show up, but the non-specific binding showed up - (reply: 2)
157. Western Blot with HPLC purified samples - non specific binding??? need help desperately (reply: 1)
158. Bradford problem in cell lysates - (reply: 1)
159. Western blotting troubleshoot - (reply: 1)
160. Signals on Molecular Weight Bands? - (reply: 1)
161. strange transfer results - (reply: 2)
162. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
163. Inconsistent Western blotting results - (reply: 3)
164. what could cause gel not to polymerize (solidify)? - gel making (reply: 8)
165. what is making my current decrease in SDS-PAGE? - SDS-PAGE Current and Resistance (reply: 6)
166. Western Blotting : Can longer incubations require more washing? - (reply: 2)
167. poor electrophoretic protein transfer - (reply: 5)
168. How to increase expression of protein in yeast? - Western from yeast and liquid beta-galactosidase assay (reply: 1)
169. Cloudy destaining solution...why! - (reply: 5)
170. DMSO in western lysis buffer - (reply: 1)
171. Wiggly 5 kDa band Western blot - (reply: 1)
172. can't view the protein band..help! - (reply: 1)
173. treatment buffer concentration - (reply: 5)
174. Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
175. Too low concentration of nuclear extract protein - after isolation (reply: 1)
176. avidin or anti-biotin - which is better? - (reply: 1)
177. combining the results from different gels in western blot - (reply: 3)
178. transfer conditions in western blot - (reply: 7)
179. Stripping of dry membrane? - can we do that? (reply: 2)
180. SDS-PAGE Basics - Help a n00bie out? (reply: 3)
152. Which quantity of purified protein in WB? - (reply: 2)
153. Sample Buffer - (reply: 1)
154. Which quantity of purified protein in WB? - (reply: 1)
155. 10% acrylamide/bisacrylamide, or 15% ? - SDS PAGE gel making (reply: 2)
156. Why my target protein did not show up, but the non-specific binding showed up - (reply: 2)
157. Western Blot with HPLC purified samples - non specific binding??? need help desperately (reply: 1)
158. Bradford problem in cell lysates - (reply: 1)
159. Western blotting troubleshoot - (reply: 1)
160. Signals on Molecular Weight Bands? - (reply: 1)
161. strange transfer results - (reply: 2)
162. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
163. Inconsistent Western blotting results - (reply: 3)
164. what could cause gel not to polymerize (solidify)? - gel making (reply: 8)
165. what is making my current decrease in SDS-PAGE? - SDS-PAGE Current and Resistance (reply: 6)
166. Western Blotting : Can longer incubations require more washing? - (reply: 2)
167. poor electrophoretic protein transfer - (reply: 5)
168. How to increase expression of protein in yeast? - Western from yeast and liquid beta-galactosidase assay (reply: 1)
169. Cloudy destaining solution...why! - (reply: 5)
170. DMSO in western lysis buffer - (reply: 1)
171. Wiggly 5 kDa band Western blot - (reply: 1)
172. can't view the protein band..help! - (reply: 1)
173. treatment buffer concentration - (reply: 5)
174. Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
175. Too low concentration of nuclear extract protein - after isolation (reply: 1)
176. avidin or anti-biotin - which is better? - (reply: 1)
177. combining the results from different gels in western blot - (reply: 3)
178. transfer conditions in western blot - (reply: 7)
179. Stripping of dry membrane? - can we do that? (reply: 2)
180. SDS-PAGE Basics - Help a n00bie out? (reply: 3)