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121. Western for a 500kda protein - some troubles, need help! - (reply: 1)
122. Mammalian Phosphatase inhibitors - (reply: 6)
123. Western blot of the supernatant - (reply: 5)
124. age of antibodies - (reply: 1)
125. Timing for cyclinD1 and c-myc - (reply: 1)
126. buffers - (reply: 1)
127. biotin-streptavidin hibridization - how can we detect biotin labeled RNAs with streptavidin HRP (reply: 2)
128. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)
129. Supernatant for western - (reply: 3)
130. using two antibodies at the same time in one blot - (reply: 4)
131. Cell lysate preparation - (reply: 2)
132. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
133. CN staining in Western blot? - (reply: 2)
134. gel and monoclonal antibody issues - (reply: 3)
135. SDS PAGE Problem - SDS page (reply: 2)
136. Tris HCl - (reply: 2)
137. Measuring intracellular protein concentration - (reply: 2)
138. What high molecular weight loading control for Western blotting? - (reply: 3)
139. Boiled samples in wrong buffer - are they beyond help? (reply: 1)
140. How to deal with insoluble proteins? - (reply: 2)
141. membrane protein and boiling - (reply: 3)
142. Alternatives to boiling SDS-PAGE samples - (reply: 9)
143. protein band is shifted after boiling sample with sample buffer - (reply: 4)
144. Western Blotting - BCIP/NBT visualization (reply: 5)
145. problem with transfer during western blot - western blot /sds page (reply: 2)
146. Is my antibody specific? - (reply: 3)
147. Yeast Western - (reply: 1)
148. Which quantity of purified protein in WB? - (reply: 2)
149. Sample Buffer - (reply: 1)
150. Which quantity of purified protein in WB? - (reply: 1)
122. Mammalian Phosphatase inhibitors - (reply: 6)
123. Western blot of the supernatant - (reply: 5)
124. age of antibodies - (reply: 1)
125. Timing for cyclinD1 and c-myc - (reply: 1)
126. buffers - (reply: 1)
127. biotin-streptavidin hibridization - how can we detect biotin labeled RNAs with streptavidin HRP (reply: 2)
128. To differentiate 53, 55 and 57kDa in the same lane (PAGE or Western) - (reply: 2)
129. Supernatant for western - (reply: 3)
130. using two antibodies at the same time in one blot - (reply: 4)
131. Cell lysate preparation - (reply: 2)
132. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
133. CN staining in Western blot? - (reply: 2)
134. gel and monoclonal antibody issues - (reply: 3)
135. SDS PAGE Problem - SDS page (reply: 2)
136. Tris HCl - (reply: 2)
137. Measuring intracellular protein concentration - (reply: 2)
138. What high molecular weight loading control for Western blotting? - (reply: 3)
139. Boiled samples in wrong buffer - are they beyond help? (reply: 1)
140. How to deal with insoluble proteins? - (reply: 2)
141. membrane protein and boiling - (reply: 3)
142. Alternatives to boiling SDS-PAGE samples - (reply: 9)
143. protein band is shifted after boiling sample with sample buffer - (reply: 4)
144. Western Blotting - BCIP/NBT visualization (reply: 5)
145. problem with transfer during western blot - western blot /sds page (reply: 2)
146. Is my antibody specific? - (reply: 3)
147. Yeast Western - (reply: 1)
148. Which quantity of purified protein in WB? - (reply: 2)
149. Sample Buffer - (reply: 1)
150. Which quantity of purified protein in WB? - (reply: 1)