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Digestion Related Discussions
  1. Double Digestion Restriction Enzyme - Troubles with double digestion (reply: 3)
  2. restriction enzyme digestion - (reply: 5)
  3. Double restriction digestion - (reply: 13)
  4. Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
  5. DNA enzyme digestion problems - Smeared and stuck in wells, no or faint bands (reply: 1)
  6. dam methylation? - digestion problem (reply: 3)
  7. genomic DNA digestion - EcoRI+MseI - (reply: 2)
  8. double digestion time - (reply: 1)
  9. Sequential digestion protocol - (reply: 3)
  10. Restriction Enzyme digestion - (reply: 4)
  11. Mysterious Band in NdeI/BamHI Double Digest - (reply: 7)
  12. failed restriction digests - (reply: 11)
  13. digestion at 4 degrees - (reply: 6)
  14. killer digest issues - (reply: 8)
  15. Xho1/Bglll digest of pSUPER.retro.puro with stuffer problems - (reply: 1)
  16. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
  17. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
  18. Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
  19. No band is visible with BamHI and XbaI Digestion - (reply: 3)
  20. Digest with BglII and SalI - (reply: 1)
  21. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  22. plasmid digestion gel - (reply: 4)
  23. restriction digestion of plasmid - (reply: 8)
  24. Genome Restriction Digestion - (reply: 2)
  25. Digest Buffer Precipitation - Na2HPO4, L-Cysteine, EDTA (reply: 4)
  26. Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
  27. Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
  28. M13 Hind and Sal digest - (reply: 2)
  29. Sequencing incorrect after digestion and ligation - (reply: 5)
  30. Digesting ethanol precipitated DNA with AseI - (reply: 2)
  31. digestion of pcr - (reply: 2)
  32. double digestion and ligation - (reply: 3)
  33. Thrombin digestion for GST - ppt problem (reply: 3)
  34. Multimerization after restriction digestion - (reply: 9)
  35. restriction enzyme digestion problem - (reply: 8)
  36. Help, sequestial digest - (reply: 1)
  37. help. Is this double digest right? - (reply: 5)
  38. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
  39. Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
  40. Double digest and 3' end polishing with klenow - (reply: 1)
  41. Mystery band after Phenol:Chloro:IAA Extraction after restriction digest - (reply: 3)
  42. how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
  43. Why does insert has two bands after RE digestion? - (reply: 3)
  44. No band after Double digestion - (reply: 4)
  45. PGEM-T easy gel - partial digestion? - (reply: 2)
  46. Miniprep DNA digestion smear - (reply: 15)
  47. only one band after double enzyme digestion - (reply: 4)
  48. all the DNA gone after digestion - (reply: 8)
  49. EcoR1-Pst1 digest for vector and insert - (reply: 3)
  50. Vector digestion - (reply: 6)
  51. how to do double digestion with EcoRI and BamHI - (reply: 9)
  52. no band after digestion - (reply: 5)
  53. BamH1-Pst1 digest - (reply: 5)
  54. problems with restriction digestion - (reply: 7)
  55. Restriciton digest protocol for adjacent sites? - (reply: 7)
  56. Ecor1/Pst1 digest - (reply: 4)
  57. For double sequential digest: which enzyme first? - (reply: 7)
  58. Problems with double digestion - (reply: 1)
  59. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
  60. can hyaluronidase be used to detach/digest cells? - (reply: 1)
  61. Dnase digestion after in vitro transcription - (reply: 4)
  62. DNA smears after digestion - DNA smears after digestion (reply: 6)
  63. RE digestion Help needed everyone - (reply: 6)
  64. P/C/I extraction of plasmid after digestion - (reply: 1)
  65. double digestion of transformed bacteria colony - (reply: 7)
  66. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  67. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
  68. Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
  69. how to check if restriction digestion has happened - (reply: 7)
  70. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  71. Digestion dilemma - (reply: 5)
  72. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
  73. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
  74. DNA degradation while digestion. - (reply: 3)
  75. HELP in Restriction Enzyme Digestion - troubleshooting re digestion (reply: 3)
  76. Proteases and thier site cleavage - Enzyme digestion (reply: 2)
  77. Digestion question - (reply: 1)
  78. Integrity of overhang after digestion - (reply: 3)
  79. PstI/SapI digestion problem - restriction enzyme (reply: 3)
  80. How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
  81. Double restriction enzyme digest - (reply: 3)
  82. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
  83. SacII digestion - (reply: 1)
  84. NotI / EcoRI digest - (reply: 5)
  85. DNA loss in digestion - I lose lot of DNA in digestion (reply: 10)
  86. pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
  87. Problems with restriction enzyme digest. - (reply: 2)
  88. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
  89. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
  90. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
  91. DNase Digestion and Reverse Transcription - (reply: 10)
  92. Double digestion - (reply: 13)
  93. double digestion - (reply: 6)
  94. unable to digest my recombinant plasmid! - (reply: 9)
  95. Double Digestion with Single Restriction Enzyme - (reply: 2)
  96. plasmid DNA Digestion - (reply: 5)
  97. NsiI digestion problems - NsiI is not working properly? (reply: 1)
  98. Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
  99. problem with restriction digestion - (reply: 3)
  100. restriction digestion - (reply: 5)
  101. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
  102. Restriction enzyme digestion problem - (reply: 4)
  103. Concerning digestion enzymes - How do digestion enzymes work really? (reply: 1)
  104. partial digestion of Tth111I - difficult??? (reply: 2)
  105. partial digestion of Tth111I - (reply: 1)
  106. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
  107. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
  108. Problem with Digestion or Ligation. How to tell? - (reply: 7)
  109. urgent HincII digestion problem - (reply: 5)
  110. Digestion in sub-optimal buffer - (reply: 5)
  111. Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
  112. Double RE digest: using two different companies - (reply: 3)
  113. Double digest question for ligation - (reply: 4)
  114. Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
  115. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
  116. Protecting against Bgl II digestion - (reply: 2)
  117. Restriction digestion - (reply: 2)
  118. Sequential digestion - (reply: 3)
  119. What is wrong with my EcoRI digestion? - Can it attribute to the star activity? (reply: 6)
  120. Can restriction digestion be replaced by CHIP? - (reply: 5)
  121. Restriction digest of pQE plasmid - Double digestion problem (reply: 7)
  122. Digestion, Ligation or transformation problem? - (reply: 8)
  123. double digestion of plasmid - problem with incomplete digestion (reply: 9)
  124. Nla III digestion - (reply: 2)
  125. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  126. Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
  127. Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
  128. digesting DNA with buffer incompatible enzymes - (reply: 4)
  129. double digestion with BamHI and HindIII - (reply: 13)
  130. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
  131. RNAse and DNA digestion in Southern Blot - (reply: 3)
  132. I need help with the steps in sequential digests please - (reply: 3)
  133. Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
  134. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
  135. Can any one help me with restriction digestion map for DNA? - (reply: 3)
  136. Is a Triple Restriction Digest Possible? - (reply: 6)
  137. Restriction digestion of PCR product - (reply: 3)
  138. AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
  139. Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
  140. ppt DNA directly from a digestion/ pcr - (reply: 2)
  141. Restriction digest - volume of restriction digest (reply: 2)
  142. RE digest and klenow - sequential blunting (reply: 6)
  143. Help with AVRII digestion - (reply: 1)
  144. which restriction enzyme should I use to digest the vector? - (reply: 3)
  145. digesting pQE30 vector gives inexplicable results - (reply: 3)
  146. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  147. Methylated DNA Interfering with Restriction Digestion - Restriction digestion problems (reply: 3)
  148. Is my restriction digest not cutting properly? - (reply: 8)
  149. can I take some of ligation mix and digest? - (reply: 4)
  150. PCR band for water but no contamination, Digestion not working - (reply: 10)
  151. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
  152. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
  153. Restriction digestion - (reply: 7)
  154. My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
  155. Transforming after digesting PCR product - (reply: 6)
  156. 3 bands after digestion - (reply: 13)
  157. Hepg2 cells resistant to trypsin digestion - (reply: 7)
  158. Restriction digestion by SpeI and SphI - (reply: 2)
  159. PacI digestion - (reply: 3)
  160. Few colonies with cloning of PCR product after digestion - (reply: 7)
  161. digesting out restriction site - (reply: 6)
  162. Religation with single digest vector - (reply: 7)
  163. Identify insert by restriction digestion - (reply: 6)
  164. problems digesting genomic DNA from cell lines - (reply: 1)
  165. Problems with SalI digestion - (reply: 5)
  166. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
  167. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  168. Tryptic digest help - Trouble diffusing stain (reply: 3)
  169. Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
  170. Problem with DNase digestion of RNA - (reply: 6)
  171. RE digest and subsequent ligation - A base is now missing from restriction site - how, why? (reply: 7)
  172. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
  173. trouble with restriction digestion - (reply: 3)
  174. BAC digestion - (reply: 8)
  175. Restriction digest as a template - (reply: 2)
  176. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  177. Problem with BamHI/SalI double digestion - (reply: 8)
  178. disappearing vector after digestion - (reply: 11)
  179. restriction digestion of PCR product? - (reply: 10)
  180. Stuborn band after digestion of plasmid DNA - (reply: 4)
  181. double digestion problem - additional band after double digestion (reply: 2)
  182. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  183. NdeI double digestion - (reply: 3)
  184. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
  185. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
  186. Full and Partial Digestion - (reply: 3)
  187. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
  188. Basic Question Restriction Digest - (reply: 11)
  189. ApaI / Not I double digestion problem - (reply: 6)
  190. Digestion Problem with BsmBI - (reply: 3)
  191. Vortexing restriction digests? - (reply: 5)
  192. If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
  193. Cloning a PCR product after digestion - (reply: 7)
  194. Digesting PCR products - (reply: 2)
  195. dimers of dna (not primers) following digest or pcr? - (reply: 11)
  196. KpnI and BamHI double digest help - (reply: 6)
  197. Pronase digestion - (reply: 1)
  198. stability of protein sample after in-gel digestion - (reply: 2)
  199. Would ExoI work? - Alternative nuclease for DNA digestion (reply: 1)
  200. How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
  201. problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
  202. keep getting incomplete digest for plasmid - (reply: 3)
  203. double digest pcDNA(+) by XhoI&XbaI, uncompletely? - (reply: 3)
  204. CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
  205. How to fill in 5` overhangs after Restriction digestion - (reply: 2)
  206. Effect of bile concentration on human digestion - Human digestion (reply: 5)
  207. restriction digestion problems - digestion problems with SalI (reply: 2)
  208. Reagents used...in restriction digestion and electrophoresis - (reply: 2)
  209. protein digestion - (reply: 1)
  210. restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
  211. trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
  212. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  213. do you sonicate or digest with lysozyme? - which one do you prefer and why.... (reply: 21)
  214. Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
  215. Lipase enzyme digestion of milk experiment - effects of stale milk - Help needed understanding how stale milk could affect my experiment... (reply: 2)
  216. Help on digestion and ligation...colonies not wanted why? - (reply: 4)
  217. problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
  218. How to extract a 20kb vector after double digestion - (reply: 4)
  219. double digestion and dephosphorylation - (reply: 5)
  220. Restriction digest - (reply: 6)
  221. Interpreting RE digest results after blunt cloning - (reply: 1)
  222. No band on double digestion of construct - (reply: 1)
  223. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
  224. BclI digestion - (reply: 4)
  225. Digestion Problem - Cloning (reply: 5)
  226. basic RE digests acting weird - (reply: 3)
  227. Nhe1 Restriction digestion - (reply: 3)
  228. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  229. tricky digestion for cloning gurus - (reply: 13)
  230. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
  231. partial digestion problem - (reply: 8)
  232. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  233. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  234. Cloning - RE digestion clean up (reply: 2)
  235. do primers and dNTPs alter digestion efficiency? - (reply: 4)
  236. Incomplete genomic digestion for Southern - (reply: 1)
  237. Plasmid appears as two bands after double digestion - (reply: 2)
  238. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  239. Partial digest to destroy an enzyme site - (reply: 10)
  240. CIP with restriction digestion - (reply: 5)
  241. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
  242. HindIII digestion - (reply: 1)
  243. Bisulfite conversion - necessary to digest DNA? - (reply: 1)
  244. how do you inactivate the enzymes after digestion? - (reply: 17)
  245. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
  246. HELP! selective scission - thrombin digestion (reply: 1)
  247. gel purification after restriction digestion - (reply: 3)
  248. what is your reaction system of digesting DNA? - (reply: 8)
  249. Digesting PCR products and cleaning up digestions - a few quick questions (reply: 4)
  250. why we use BVS in DNA digestion? - (reply: 3)
  251. Retroviral Vector won't digest ideas! - (reply: 1)
  252. restriction digestion problem... - (reply: 12)
  253. unpredictable plasmid - can digest but not sequence. . . (reply: 4)
  254. Problem with BsaB1 digestion - (reply: 1)
  255. how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
  256. Southern - Southern Blot Digestion (reply: 4)
  257. restriction digest and DNA degradation - (reply: 3)
  258. what's wrong with my digestion - (reply: 2)
  259. Mystery band after vector digestion - (reply: 3)
  260. digestion with two REs requiring different buffer - (reply: 10)
  261. Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
  262. Problem with restriction digestion - (reply: 10)
  263. problems with digesting insert from vector - (reply: 4)
  264. How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
  265. restriction digestion - (reply: 2)
  266. T4 blunting follow by digestion - (reply: 2)
  267. Question on digestion - (reply: 4)
  268. digestion with pvu1 enzyme - (reply: 3)
  269. genomic DNA PCR - RE digestion of gDNA before amplification (reply: 3)
  270. Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
  271. double digestion - Time for digestion (reply: 1)
  272. Double digestion/ligation problem - (reply: 4)
  273. restriction enzyme digestion problem - (reply: 7)
  274. Can I directly digest PCR products? - (reply: 9)
  275. Restriction Digest Woes - (reply: 1)
  276. Question about NcoI digestion... - (reply: 3)
  277. double digest not working? - BamHI+SacI double digest (reply: 4)
  278. Double digestion of PCR product and ligation - (reply: 5)
  279. what's wrong? Plasmid digestion produce only smear - (reply: 6)
  280. Dnase I digestion - (reply: 3)
  281. Plasmid digestion - Problem with digestion (reply: 3)
  282. Double digestion problem - (reply: 1)
  283. troubleshooting Rsa-I restriction digests - (reply: 2)
  284. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  285. digestion problem - incomplete digestion of transformed plasmid (reply: 1)
  286. problem with generation of restriction digestion site - (reply: 4)
  287. Digest, refill an digestion - How can I do it? (reply: 2)
  288. maximum [EDTA] in restriction digests - (reply: 2)
  289. tRNA and digestion - (reply: 1)
  290. Double Digestion - double digestion for southern (reply: 1)
  291. double digestion - double digestion (reply: 2)
  292. in- gel trypsin digestion - (reply: 1)
  293. DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
  294. digestion problems - (reply: 1)
  295. Dodgy Digest - (reply: 3)
  296. double digestion problem - (reply: 9)
  297. Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
  298. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
  299. problems purifying plasmid DNA after restriction digestion - (reply: 3)
  300. Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
  301. DNA precipitated out in digest reaction - (reply: 1)
  302. Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
  303. BamH1 and Xho1 double digestion problem - digestion problem (reply: 7)
  304. Genomic DNA Complete Digestion - (reply: 4)
  305. digest problem - extra bands (reply: 2)
  306. Problems digesting with BclI and SspI - Any help is apriciated (reply: 1)
  307. Enzyme digestion before bisulfite treatment - (reply: 1)
  308. DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
  309. Double digest with EcoR1 - would it work? - (reply: 1)
  310. partial digestion - match partial digestion conditions (reply: 1)
  311. Online tool for restriction digestion of DNA - (reply: 5)
  312. BamH1 and Nde1 double digestion problem - (reply: 3)
  313. After restriction digestion my bands just disappear - (reply: 3)
  314. restriction digestion - (reply: 2)
  315. A Digestion problem - (reply: 6)
  316. Double Digest problems - (reply: 2)
  317. Digestion time and enzyme - (reply: 2)
  318. digestion & purification problem - (reply: 6)
  319. Proteinase digestion of tissue for DNA extraction - (reply: 3)
  320. genomic DNA digest - (reply: 3)
  321. Methylation and restriction digestion problem - (reply: 8)
  322. Double digestion and ligation problem - (reply: 15)
  323. Phages - How to digest lambda DNA (reply: 1)
  324. Restriction digestion problem!! - XbaI digestion (reply: 1)
  325. Restriction digest - (reply: 1)
  326. DNA digestion time? - how long how many units (reply: 1)
  327. Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
  328. enzymatic digestion - (reply: 3)
  329. Cant digest my vector - Enzyme digest failing (reply: 5)
  330. Difficult double digestion - (reply: 3)
  331. The detailed digestion site map of pCO1 or pHaMDR1/A - (reply: 1)