Would ExoI work? - Alternative nuclease for DNA digestion (Apr/17/2006 )
Did anyone try to use a different enzyme, instead of P1 for DNA digestion for total methyl cytosine quantification assays before?
I mean from what i read here, it sounds like, P1 works on single-stranded DNA, and so is ExoI and it is somewhat cheaper and more accessible than P1.
-L_Han-
be sure to denature your DNA and then snap chill before adding your nuclease as this increases the liklihood of single stranded DNA for nuclease digestion.
I have seen ExoI being used as well and can't really see anything wrong with using it, we are using P1 btw.
Nick
-methylnick-