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Purification Related Discussions
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Virus vs protein purification - (reply: 2)
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How to eliminate inherent proteins contamination during protein purification? - (reply: 1)
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plasmid purification - (reply: 4)
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Protein purification questions - (reply: 3)
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Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
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low copy number plasmid purification kit - (reply: 4)
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Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
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Virus vs protein purification - (reply: 3)
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BAC DNA purification - (reply: 4)
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How to make antibody based affinity column for protein purification - (reply: 4)
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protein purification from agarose beads using immunoprecipation kit - (reply: 2)
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Fab antibody fragment purification - (reply: 2)
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Purification of linearized AT-rich DNA for transfection - (reply: 4)
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Plasmid purification for Gibson Assembly - (reply: 2)
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HSP70 contamination after Nickel purification - (reply: 3)
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Doubt on HisTag protein purification miniprep - (reply: 3)
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troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
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Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
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Affinity purification glycine elution chemistry - (reply: 3)
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storage conditions for purified protein - (reply: 2)
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large scale protein purification - (reply: 6)
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Gel Purification: When is it safe to pause? - (reply: 2)
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after protein purification - (reply: 4)
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Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
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Cloning purified PCR product into cells - (reply: 5)
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antibody/DNAconjugation and purification - (reply: 1)
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Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
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Question about MBP purification - (reply: 2)
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RNA purification with TRI_ low yield - (reply: 1)
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Why I’m not getting any band after column purification? - (reply: 1)
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Proteinase extraction and purification - (reply: 1)
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Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 4)
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Purification of metallothioneins? - (reply: 1)
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Low 260/230 ratios from DNA purification question - (reply: 1)
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ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
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Please help me :Protein expression and purification - (reply: 1)
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Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
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Bacterial DNA extraction/purification from animal tissue - (reply: 1)
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DNA virus purification from agarose failed! - (reply: 7)
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Any real difference between DNA purification kits? - (reply: 9)
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how much purify protein nedded for IP - (reply: 9)
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regarding protein purification using 8m urea and sds page - (reply: 2)
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Protein Purification from mammalian cells - (reply: 1)
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Which method for DNA purification b4 cloning - (reply: 4)
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Purification for TOPO - (reply: 1)
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Purified DNA fragments are bigger than unpurified - (reply: 2)
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purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
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his-tag purification - (reply: 9)
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IgG purification from hybridomas - (reply: 2)
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DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
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faint dna band after pcr purification - (reply: 1)
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transformation and protein purification calculation help; very basic - (reply: 5)
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PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
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Plasmid digestion and gel purification using HindIII - (reply: 3)
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Do protein purification under partially denatured condition - (reply: 1)
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Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
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Gel purification (DNA) - (reply: 1)
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Viral contamination in DNA purification columns - (reply: 1)
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Plasmid Purification Using an Alkaline Lysis - (reply: 1)
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Question about DNA purification from a restriction digestion reaction - (reply: 1)
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Protein Purification-catastrophe while purifying? - (reply: 2)
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What is the membrane made of in DNA purification kits - (reply: 3)
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Protein purification for adherent mammalian cells - (reply: 2)
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pGEX-2t purification problem - (reply: 3)
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Problem with strep tagged protein purification - (reply: 5)
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protein purification in acanthamoeba lysate - (reply: 3)
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Polyclonal Ab purification - (reply: 1)
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purify protein from native gel - (reply: 1)
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purifying proteins with repeats - (reply: 3)
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PCR RFLP is PCR product purification necessary? - (reply: 3)
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Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
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Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
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Streptavidin purification in the lab - (reply: 4)
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adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
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Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
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No DNA after PCR product purification - (reply: 9)
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removal of ethanol in PCR product purification - (reply: 5)
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Trouble with purified recombinant protein bands - (reply: 3)
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Protein activation after purification - (reply: 4)
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Contaminants in protein purification.....help! - (reply: 3)
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Protein expression and purification - Please help - (reply: 2)
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Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
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tag for protein purification - (reply: 7)
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Low yield PCR product after gel purification - (reply: 8)
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Trouble in GST- fusion protein purification - (reply: 1)
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low concentration protein purification - (reply: 1)
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Calculating amount of Beads for purification of Ig - (reply: 3)
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Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
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PCR product purification - (reply: 4)
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EMSA: cell extract vs purified protein - (reply: 2)
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Protein purification method to isolate elicitor with biological activity - (reply: 1)
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purification and binding of protein in denaturing conditions - (reply: 2)
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problem with Enzyme purification - (reply: 4)
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Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
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Protein Purification from Polyacrylamide Gels - (reply: 3)
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Do you have to purify target vector after double digest from MCS piece - (reply: 1)
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Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
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Purifying a "protein" - (reply: 2)
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Help! Purifying native protein-2 years and counting... - (reply: 4)
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Large scale Viral RNA purification Kit - (reply: 3)
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phage lysate purification - (reply: 2)
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the virus particles purification methods - (reply: 7)
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Silanized equipment when purifying tRNA? - (reply: 1)
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Plasmid Purification and Digestion Problem - (reply: 2)
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Purification of FC tagged proteins - (reply: 3)
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Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
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GST fusion protein purification - (reply: 2)
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Why do i get DNA of low concentrate after purification with kit - (reply: 9)
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pcr purification - (reply: 1)
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pcr purification doubt - (reply: 4)
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Mouse IgG1 purification - Protein G - (reply: 1)
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How to purify antioxidants enzymes from plant tissue. - (reply: 1)
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pcr purification - (reply: 1)
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Use PCR purification kit to purify restriction digestion - (reply: 2)
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Help with His-Tag protein purification - (reply: 2)
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protien purification using flag affinity - (reply: 4)
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SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
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MW of PEG for Purification of DNA - (reply: 2)
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HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
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Reversed-Phase chromatography for protein purification - (reply: 3)
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Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
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problem with purification of gst-tagged protein - (reply: 2)
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Saliva and GCF DNA purification - (reply: 1)
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DNA isolation/purification troubleshooting. - (reply: 5)
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How to purify RNA from a handful number of cells? - (reply: 5)
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RNA purification from urine samples - (reply: 2)
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Purifying recombinant protein from pellet? - (reply: 2)
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Purifying RNA from Solution with RNEasy? - (reply: 2)
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Columns for ssDNA oligo purification - (reply: 2)
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low concentration of purified PCR product - (reply: 2)
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5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
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what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
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Extra bands after protein expression/purification - (reply: 4)
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PCRed on restriction sites -- how do I purify it? - (reply: 8)
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purification of digested vector by agarose eletrophoresis problem - (reply: 4)
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My product of PCR haven't the lenght right after purification....... What - (reply: 2)
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Troubleshooting for DNA purification - (reply: 1)
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Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
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Insoluble Protein purification - (reply: 1)
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How to purify unstable protein? - (reply: 10)
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Purification of oligos for EMSA - (reply: 1)
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Hisx18 purification - (reply: 4)
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Homemade MiniPreps for Plasmid Purification - (reply: 1)
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getting rid of imidazole in purified protein? - (reply: 2)
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BN-PAGE of purified membrane associated protein - (reply: 5)
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Anion exchange purification - (reply: 3)
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Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
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Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
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LDH purification - (reply: 1)
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NO yield after purification using Qiagen kits - Please help! - (reply: 1)
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A problem with IgG purification by Protein G chromatography - (reply: 4)
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Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
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Help: I am loosing the DNA during PCR purification - (reply: 7)
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FACS Sorting and Protein Purification - (reply: 5)
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His-Taq purification - (reply: 4)
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Protein purification buffer - (reply: 3)
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Protein purification in native conditions - (reply: 1)
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Butyl-agarose/sepharose purification - (reply: 1)
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methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
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type of IgG in my sample?? - proteinA purified sample (reply: 1)
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Help with small protein with his-tag purification - (reply: 7)
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Purification of DNA after MeDIP and WGA - (reply: 1)
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Protein purification from HEK 293 cells - (reply: 1)
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~200kb Plasmid DNA purification is not working - (reply: 1)
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how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
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protein purification - (reply: 1)
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plant protein purification opinion - (reply: 3)
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Unable to Purify protein! - Protein purification (reply: 2)
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Problem with Pierce GST spin purification column - (reply: 6)
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HIS-tagged protein purification - (reply: 12)
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His-tagged protein purification problem - (reply: 2)
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Inclusion body purification - (reply: 1)
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Purification of recombinant proteins from insect cells - (reply: 2)
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Nuclear or Cytoplasmic RNA Purification - (reply: 1)
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No DNA after PCR purification with QiaQuick? - (reply: 4)
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DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
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Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
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Multiple bands from purified PCR product - (reply: 3)
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Troubleshooting Protein Purification using FPLC - (reply: 1)
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protein yield is very low after purification - protein purification (reply: 9)
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His tag protein purification - (reply: 7)
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Help with GST protein purification - (reply: 4)
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Purification of His tagged protein - (reply: 2)
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Protein purification. How to make function of recombinant protein? - (reply: 2)
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Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
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purification of PCR product for cloning in a vector - (reply: 3)
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His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
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purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
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His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
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plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
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Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
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Really strange problem with sequencing and plasmid purification! - (reply: 8)
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Decrease in concentration after protein purification - (reply: 3)
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inactive enzyme from purification - (reply: 1)
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strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
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His-tagged protein purification - (reply: 3)
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protein purification - (reply: 3)
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Purification od insoluble protein (TIMP3) - (reply: 2)
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white residue after DNA purification - (reply: 3)
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Problems with qiaquick gel purification kit - cloning (reply: 10)
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problem in protein purification - (reply: 5)
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protein purification - (reply: 5)
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Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
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Problem with protein purification By FPLC - (reply: 4)
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uncut vector contamination in gel purified band - (reply: 2)
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Purify Chlorella Sample - (reply: 3)
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Purified lambda phages - (reply: 1)
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About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
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DNA purification from blood - (reply: 6)
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Preparation of Purified Inclusion Bodies - (reply: 2)
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salt for DNA precipitation - DNA purification (reply: 3)
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DNA quantification after gel purification - (reply: 6)
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Protein Purification by precipitation - (reply: 2)
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Problem with MBP and GST tag purification - (reply: 1)
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Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
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Protein TAG for affinity purification - (reply: 1)
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M2 Flag purification - (reply: 2)
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Recombinant protein aggregation after purification - (reply: 2)
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best and simple method to purify protein - (reply: 38)
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strange problem with His purification - (reply: 1)
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ChIP purification method - (reply: 7)
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inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
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antibody purification - ammonium sulfate precipitation - (reply: 7)
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Problem with Histag purification - (reply: 1)
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How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
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Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
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IMPACT system purification problem - (reply: 1)
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additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
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Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
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Purification of Protein Extract for ELISA - (reply: 1)
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Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
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Help...purify and concentrate protein for IEF - (reply: 1)
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NI-NTA purification - (reply: 2)
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What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
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DNA purification - DNA purification dissolved in water (reply: 3)
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purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
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Purification problems his-tag - I have tried everything (reply: 3)
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weak purified antibody - (reply: 4)
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after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
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Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
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His Tag Protein Purification - (reply: 6)
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Inclusion Body purification - (reply: 2)
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membrane protein purification - (reply: 6)
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Inseparable insert in gel purification of the vector - (reply: 3)
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Contamination in GST fusion protein purification - (reply: 2)
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Purification of scretory protein from bacteria - (reply: 2)
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Ni-NTA his tag protein purification - (reply: 1)
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Tips for antibody purification from crude serum? - (reply: 3)
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Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
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Problems with affinity purification with M2 beads - (reply: 2)
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plasmid purification kit for transfection - (reply: 2)
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best plasmid purification kit for transfection - (reply: 1)
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Purify ChIP DNA using Qiagen spin filter - (reply: 8)
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Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
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problem in the GST fusion protein purification - (reply: 4)
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Troubleshooting GST purification - (reply: 4)
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Storing Purified Proteins - Seeking a Preservative (reply: 6)
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Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
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purification of a large amount of DNA - (reply: 3)
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Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
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affinity purification and proteomics - (reply: 1)
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protein is degraded after purification - (reply: 2)
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problem in pcr and purification - (reply: 3)
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exosome purification - (reply: 2)
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Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
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PCR product purification - (reply: 3)
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Protein purification from Insect media - (reply: 1)
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Purifying a strange native protein - (reply: 6)
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Purification of virus particles - (reply: 2)
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want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
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Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
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Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
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Couple questions about GST purification - (reply: 16)
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Rabbit IgG purification - Rabbit IgG purification (reply: 5)
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Trypsin Expression and Purification - (reply: 3)
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Purifying and cloning 69nt product - (reply: 5)
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ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
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dnase treatment before his-tag purification - (reply: 3)
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vortexing after phenol-chloroform-IMA purification - (reply: 3)
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His tag protein purification - (reply: 3)
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GST-fusion protein purification - How to purify it? (reply: 9)
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Gel Purification problem..Help~! - (reply: 7)
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mAb purification but precipitated - (reply: 13)
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Ab purification - (reply: 1)
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restriction digestion of PURIFIED PRODUCT - (reply: 5)
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Problems in His-tag protein purification with TALON - (reply: 1)
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Endogenous protein purification - (reply: 2)
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purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
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Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
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PCR and gel purification problem - did I screw this up (reply: 4)
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Purifying hydrogen bonded peripheral protein - (reply: 3)
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GST-tag protein purification - GST-tag protein purification (reply: 4)
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Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
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How to purify a transmembrane protein from others proteins? - (reply: 1)
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SDS Page Help - using purified protein (reply: 3)
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GST fusion protein purification - (reply: 1)
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Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
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B-per GST fusion protein purification kit - (reply: 2)
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Principles behind DNA RNA protein isolation and purification - (reply: 2)
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Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
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A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
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how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
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a doubt about PCR gel purification - (reply: 4)
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Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
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Problem with DNA purification from agarose gel - (reply: 3)
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DNA purification - long fragments wanted - (reply: 1)
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purification of a basic protein - problem in elution (reply: 4)
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stabilization of purified protein solutions - (reply: 2)
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vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
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silica purification - problem with double bands after silica purification (reply: 1)
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Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
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How to purify a protein (PI 8.0) using His tag? - (reply: 4)
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Gel purification of vector DNA - low yield - (reply: 26)
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gel purification or ligation problem - (reply: 3)
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Help with Purifying DNA Hairpin - (reply: 2)
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Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
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protein purification - (reply: 5)