Who can advise me a kit that would allow me to purify amplicons from quantitativ - (Feb/09/2015 )
I wish to sequence the bacterial DNA amplification products amplified by real-time quantitative pcr (SYBR Green method) for that I need a kit that would allow me to purify the amplified amplicons, so who can give me a name of a kit that would allow me to accomplish this task ? Thanks
-mer-
Any normal pcr cleanup process will work for q-pcr cleanup. Nothing special is needed.
-phage434-
I use this one form Favorgen that is the cheapest in market:
GEL/PCR Purification Mini Kit (300prep)
Code:
FAGCK001-1
Supplier:
Favorgen Biotech Corporation
Size:
300 Prep
BUY ONE GET ONE FREE: FAGCK001-1 AND FAPDE300
$141.00 CDN
-BMF-
I've never heard of this company, but any kit of this kind will likely work. You could even likely just do an ethanol precipitation, which would be much cheaper.
-phage434-