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Cryopreservation Related Discussions
  1. Cell pellet after freezing - (reply: 3)
  2. THP-1 thawing and culturing protocol - (reply: 1)
  3. thaw samples? - (reply: 6)
  4. Retrovirus freezing - (reply: 2)
  5. cell cryopreservation in -80 - (reply: 2)
  6. glycerol as a protectant during freezing? - workingprinciple (reply: 7)
  7. Freezing mESC - (reply: 2)
  8. Freezing medium - (reply: 5)
  9. Very high confluence after thawing cells– good or bad? - (reply: 8)
  10. Protein expression changes after freezing+thawing - (reply: 1)
  11. Problem with thawing cells from liquid nitrogen? - (reply: 6)
  12. Freezing splenocytes - (reply: 3)
  13. Restaining cells after cryopreservation - (reply: 7)
  14. Freezing cells before lysis? - (reply: 4)
  15. THP-1 cell culture problem: Cells die after thawing - (reply: 4)
  16. Freezing transformations? - (reply: 1)
  17. time required for cell recovery after thawing - (reply: 8)
  18. plasmid concentration and freezing - (reply: 4)
  19. Snap freezing tissue for RNA isolation - (reply: 10)
  20. Ooops! I messed up! Help! - freezing cells (reply: 5)
  21. Schock freezing - cell disruption? - (reply: 3)
  22. cryopreservation of lymphocytes - (reply: 13)
  23. Passaging stable transfected cells after thawing - (reply: 4)
  24. thawing frozen tissue and culturing - (reply: 1)
  25. freezing cells in log phase - (reply: 2)
  26. lost of enzyme activity after thawing - (reply: 3)
  27. why my IMEM looks a bit yellow after thaw? - (reply: 1)
  28. will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
  29. Freezing Lysates before Sonication - (reply: 1)
  30. Freezing cells in blocking buffer? Urgent! - (reply: 7)
  31. freezing of primers - (reply: 2)
  32. How to remove DMSO after thawing? - (reply: 4)
  33. Why freeze slowly and thaw quickly? - (reply: 5)
  34. freezing HEK293 cells - (reply: 3)
  35. rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
  36. Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
  37. thawing in -20C for overnight - is this harmful (reply: 6)
  38. cryopreservation agent missing in frozen cell stock - (reply: 3)
  39. Freezing and thawing - Good thawing V% but...... (reply: 4)
  40. freezing PBMC - (reply: 4)
  41. Cells after thawing - (reply: 6)
  42. After thawing; attached then detached - (reply: 3)
  43. Shall we thaw fast or slow? - (reply: 8)
  44. Slow cell growth when thawing cells - (reply: 7)
  45. Could we freeze the 0.25 trypsin EDTA and thaw it, use it? - (reply: 8)
  46. Maintaining cells without freezing - (reply: 5)
  47. thawing of fridged cell culture - Reviving of cells (reply: 2)
  48. Freeze/Thaw procedure in cell lysis - How is this done??? (reply: 12)
  49. cells not adhering to flask after thawing - (reply: 14)
  50. RNA extraction - freezing homogenate - (reply: 1)
  51. Thawing on ice necessary? - (reply: 8)
  52. Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
  53. Cryoprotectants --> Freezing Bacterial Cells - Help with cryoprotectants in freezing E. coli. (reply: 4)
  54. Recovery of HeLa cells - after thawing recovery is 0% (reply: 4)
  55. A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
  56. Freezing a Pellett.. (or however its spelt) - Spin laballed over expressed protein (reply: 2)
  57. Slow freezing and thawing of FCS - (reply: 1)
  58. Freezing and thawing primary lymphocytes - Any protocols? (reply: 1)
  59. Freezing cells-a problem! - (reply: 2)
  60. Freezing cells - (reply: 2)
  61. freezing medium - how long can u store it? (reply: 2)
  62. Freezing TCA precip pellet? - (reply: 3)
  63. Cell clumping during thawing - (reply: 2)
  64. G418 in freezing medium? - (reply: 2)
  65. why did precipitate formed after antibody freez/thaw ? - (reply: 2)
  66. Freezing method for Hybridoma - (reply: 1)
  67. Rack for cryopreservation - Question regarding racks for cryopreservation (reply: 1)
  68. how to thaw intestinal samples? - (reply: 1)
  69. How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
  70. cryopreservation and nitrogen - cells in vapors or in liquid (reply: 8)
  71. Freezing medium and cell counting - (reply: 6)
  72. Thawing of competent bacterial cells - (reply: 2)
  73. freezing of cells - (reply: 9)
  74. How to get rid of OCT used in freezing section - Method (reply: 2)
  75. Need help on thawing primary cells - Cells dead!!! (reply: 7)
  76. Why use DMSO and FCS for freezing cells? - (reply: 5)
  77. Freezing HeK'n cells - (reply: 2)
  78. stable siRNA - no downregulation after thawing (reply: 1)
  79. Freezing down cell lysate - (reply: 2)
  80. LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
  81. Freezing and thawing of cells - (reply: 112)
  82. cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
  83. help with cryopreservation - (reply: 25)
  84. Degraded cDNA after freezing - (reply: 2)