Freezing mESC - (May/29/2008 )
Hi!
I was suppose to write in the other topic, but I found it didn't have much to do with that. So, for how many passages can I culture cells before freezing them for post-use? And what freezing medium would you recommend? I've tried using Bambanker at an early passage and it worked well, despite once it didn't work.
Thanks!
-Doda-
QUOTE (Doda @ May 30 2008, 01:47 AM)
Hi!
I was suppose to write in the other topic, but I found it didn't have much to do with that. So, for how many passages can I culture cells before freezing them for post-use? And what freezing medium would you recommend? I've tried using Bambanker at an early passage and it worked well, despite once it didn't work.
Thanks!
I was suppose to write in the other topic, but I found it didn't have much to do with that. So, for how many passages can I culture cells before freezing them for post-use? And what freezing medium would you recommend? I've tried using Bambanker at an early passage and it worked well, despite once it didn't work.
Thanks!
there is no really a limit for the passage number but you want to keep it as low as possible. For freesing media I use 40%FBS, 50% media, 10%DMSO.
-cmonet-
Thanks again, cmonet!
Next time I'll be trying this.
-Doda-