thawing in -20C for overnight - is this harmful (Nov/30/2006 )

hi all, i just want to ask that i have kept the cells for thawing in -20C for overnight, whether this will harm the cells?

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I dont think that thawing will harm your cells or significantly reduce viability of your cells.

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is it bacteria or eucaryotes? was freeze medium still frozen or liquid? for bacteria I don´t mind, but eucaryotes may be critical, some are definitely sensitive

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I agree with Kosmodrom, I would not do it for eucaryotes.
bacteria are much more resistant.

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Always freeze cells as slowly as you can and do the opposite for thawing.

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i wonder why you thaw cells in -20C ,most of us do this at 37-40C.

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Depending on what type of cells you work with. I know for embryonic stem cells, thawing is performed at 37C and as quickly as possible. Thawing slowly for prolonged time is to be avoided.

--Jessica

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