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Immunology-and-Histology
91.
Any Specific Vital Stains, Dyes? -
(reply: 1)
92.
Staning macrophages for FACS -
(reply: 1)
93.
Immunofluorescense of floating cells - How to IF the floating cells during apoptosis?
(reply: 3)
94.
NK Cell Isolation - negative selection kits -
(reply: 1)
95.
Beta galactosidase labbeling -
(reply: 3)
96.
IL4 and GMSCF for DC culture - how much IL4 and GMSCF for culturing dendritic cells
(reply: 2)
97.
Macrophage fixation for immunofluorescence - Sucrose yes or sucrose not
(reply: 3)
98.
Sectioning small pieces of tissue - How to section insect brain?
(reply: 9)
99.
T cell activation for intracellular cytokine detection by Flow Cytometry -
(reply: 1)
100.
nuclear staining without permeablization -
(reply: 3)
101.
Control for primary antibody in Immunohistochemistry - What is it?
(reply: 4)
102.
TRAP staining - TRAP staining of osteoclasts in vitro
(reply: 3)
103.
IP buffer RIPA - Preserve buffer
(reply: 3)
104.
IHC on the liver: any special tricks needed? -
(reply: 1)
105.
Phosphatase treatment as a control for Phospho antibodies -
(reply: 1)
106.
Cell death after PMA treatment -
(reply: 5)
107.
FITC Labelling -
(reply: 1)
108.
Basic immuno/histo common knowledge question - Where can I find what cells in what organ?
(reply: 4)
109.
MHC II molecules - antigen bound state - purchasable conformation specific antibodies
(reply: 1)
110.
immunohistochemistry high backgroungd staining -
(reply: 6)
111.
Immunohistochemistry in human skin tissue -
(reply: 1)
112.
how to distinguish monocyte and dendritic cell under microscope -
(reply: 2)
113.
ELISA - how to make a protein lysate? -
(reply: 3)
114.
How long tissue can stary in sucrose or fixative before cryosectioning? -
(reply: 5)
115.
software for overlapping IF images -
(reply: 5)
116.
Immunohistochemistry blocking - Whether to use or not?
(reply: 1)
117.
What immuno-method to use? -
(reply: 9)
118.
LPS -
(reply: 4)
119.
ELISA standards. -
(reply: 3)
120.
phalloidin and methanol -
(reply: 1)
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