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Immunology-and-Histology
1021.
FLAG Peptide how much to use w/M2 clone - competition w/M2 clone for immunocyto
(reply: 1)
1022.
Self-purified antibody - viability and quantity problem -
(reply: 1)
1023.
coating microplates for ELISA - Questions about coating
(reply: 8)
1024.
No signal of my normalisers - Western blot
(reply: 3)
1025.
all intensities disappear after stripping - Western blot
(reply: 2)
1026.
HRP conjugation - Ag-HRP conjugation
(reply: 2)
1027.
Antibody Viability - are my antibodies alive??
(reply: 3)
1028.
Alkaline Phosphotase substrate for ELISAS -
(reply: 2)
1029.
Fixation for IF??? - best method for nuclear antigens
(reply: 1)
1030.
Flow cytometry - permeabilization protocol? -
(reply: 1)
1031.
Problem with expression of marker gene -
(reply: 1)
1032.
Immunofluorescence - Question about storing cells grown on slides
(reply: 4)
1033.
Optimizing ELISA! - Papers and help on Optimizing my ELISA!!
(reply: 1)
1034.
GST-recomb-fusion-protein antibody - antibody against gst-fusion protein
(reply: 1)
1035.
Natural tissue affinity of AEC used in IHC - AEC is a better substrate in immunoperoxidase test
(reply: 1)
1036.
after de-parafinization and hydration - future IHCs
(reply: 1)
1037.
ELISA beginner - bacterial protein
(reply: 2)
1038.
ELISA Plates - comparison of OD
(reply: 3)
1039.
Cover slip for confocol?? -
(reply: 5)
1040.
Fixation method for confocal microscopy -
(reply: 4)
1041.
Tissue staining problem with polyclonal antibody -
(reply: 3)
1042.
one gene, 2 variants - which one is relevant?
(reply: 2)
1043.
mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS
(reply: 1)
1044.
How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein
(reply: 3)
1045.
BSA -
(reply: 4)
1046.
Interpreting ELISA results -
(reply: 2)
1047.
EGFP detection -
(reply: 4)
1048.
Staining HT29 cells with TLR Abs and FITC, TRITC and DAPI -
(reply: 6)
1049.
Statistics for interpreting ELISA results - Statistics for interpreting ELISA results
(reply: 2)
1050.
Problem with phalloidin staining -
(reply: 2)
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