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1021. FLAG Peptide how much to use w/M2 clone - competition w/M2 clone for immunocyto (reply: 1)
1022. Self-purified antibody - viability and quantity problem - (reply: 1)
1023. coating microplates for ELISA - Questions about coating (reply: 8)
1024. No signal of my normalisers - Western blot (reply: 3)
1025. all intensities disappear after stripping - Western blot (reply: 2)
1026. HRP conjugation - Ag-HRP conjugation (reply: 2)
1027. Antibody Viability - are my antibodies alive?? (reply: 3)
1028. Alkaline Phosphotase substrate for ELISAS - (reply: 2)
1029. Fixation for IF??? - best method for nuclear antigens (reply: 1)
1030. Flow cytometry - permeabilization protocol? - (reply: 1)
1031. Problem with expression of marker gene - (reply: 1)
1032. Immunofluorescence - Question about storing cells grown on slides (reply: 4)
1033. Optimizing ELISA! - Papers and help on Optimizing my ELISA!! (reply: 1)
1034. GST-recomb-fusion-protein antibody - antibody against gst-fusion protein (reply: 1)
1035. Natural tissue affinity of AEC used in IHC - AEC is a better substrate in immunoperoxidase test (reply: 1)
1036. after de-parafinization and hydration - future IHCs (reply: 1)
1037. ELISA beginner - bacterial protein (reply: 2)
1038. ELISA Plates - comparison of OD (reply: 3)
1039. Cover slip for confocol?? - (reply: 5)
1040. Fixation method for confocal microscopy - (reply: 4)
1041. Tissue staining problem with polyclonal antibody - (reply: 3)
1042. one gene, 2 variants - which one is relevant? (reply: 2)
1043. mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
1044. How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein (reply: 3)
1045. BSA - (reply: 4)
1046. Interpreting ELISA results - (reply: 2)
1047. EGFP detection - (reply: 4)
1048. Staining HT29 cells with TLR Abs and FITC, TRITC and DAPI - (reply: 6)
1049. Statistics for interpreting ELISA results - Statistics for interpreting ELISA results (reply: 2)
1050. Problem with phalloidin staining - (reply: 2)