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781. No p53 in the HEK293 cells on my IF - Fluorescence microscopy (reply: 4)
782. Is it possible: Effect of OVA on murine BMDC phenotyp ?! - (reply: 4)
783. CD system of leukocyte surface molecules - (reply: 2)
784. puncta staining problem - (reply: 5)
785. T cell proliferation assay - (reply: 3)
786. frozen tissue prep for IHC and westerns - (reply: 1)
787. Human Active MMP-8 ELISA - (reply: 1)
788. Tetramers - (reply: 3)
789. Monoclonal Ab is always from mice? - (reply: 3)
790. Antibodies to estrogen receptor, recommendations? - (reply: 1)
791. Annexin staining - strange & mysterious population - (reply: 3)
792. plate bound vs. soluble anti-CD3 - (reply: 2)
793. FACS of stimulated human PBMC - (reply: 2)
794. microtubule imaging - (reply: 6)
795. flow cytometry - labelling surface receptor on monolayer cells (reply: 5)
796. Direct Immunofluorescence Protocol - (reply: 3)
797. two mouse antibodies can be stained with different secondaries? - different colors? (reply: 3)
798. Unequal signal intensity across Western blot membrane - (reply: 4)
799. How to strip PVDF membrane after imaging ? - For western blot (reply: 2)
800. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
801. Imaging fixed cells - Immunofluorescence (reply: 6)
802. debris in immunocytochemistry... - how to get rid of them???? (reply: 3)
803. How to detect the antigenicity of peptide? - except ELISA (reply: 7)
804. how to reduce the background in the ELISA assay? - using peptide as antigen (reply: 18)
805. Who sells good primary antibodies for Westerns? - Who's your favourite supplier? Least favourite? (reply: 24)
806. how can i preserve the purified mAb for a long time? - (reply: 7)
807. Isolate splenocytes from rat - (reply: 2)
808. FACS and isotypes - (reply: 3)
809. preparing peritoneal macrophage - problem in lysing erytrocyte (reply: 3)
810. Magnetic particle ELISA - few questions - Seems to work well, but not being used much?? (reply: 3)