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241. immunohistochemistry and protein denaturation - (reply: 3)
242. How Conserved are the "Constant" regions of 'IgG' Heavy and Li - (reply: 5)
243. CD31 Staining of PFA fixed Frozen Tissue - (reply: 1)
244. Blood sample preparation. - (reply: 1)
245. polyclonal antibodies using inclusion bodies - how to prepare sample (reply: 1)
246. Western vs. ELISA - (reply: 5)
247. Can I use DAPI and GFP at the same time? - Need advice on fluorochromes for confocal microscopy (reply: 8)
248. labelling primary antibody with fluorescent tag - (reply: 1)
249. empty vector control - (reply: 4)
250. Anti-rabbit Cy3 - (reply: 3)
251. Good mounting medium - (reply: 4)
252. Hybridoma culture not secreting mouse antibody - (reply: 6)
253. Is GPF/YFP signal OK after fixation? - Does fixation for immunofluorescent stain cause loss of GPF/YFP signal (reply: 6)
254. IHC - No staining whatsoever - (reply: 8)
255. Intracellular IL2? - (reply: 4)
256. problem with the mititracker staining - (reply: 3)
257. question about penicillin allergy - (reply: 12)
258. how to distinguish normal and cancerous osteoblast cells - (reply: 3)
259. Phalloidin staining - problem - (reply: 13)
260. T-cell proliferation assay with soluble OKT3 - (reply: 1)
261. Non toxic H&E staining? - (reply: 1)
262. Role of glycine based blocking in Immunofluorescence - (reply: 4)
263. rat bone marrow mast cell maturation - Anyone have any experience or advice? (reply: 1)
264. protein G, binding buffer pH? - (reply: 3)
265. Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
266. Optical density variation in indirect ELISA - (reply: 3)
267. Which p-Serine antibody is suitable for IP and WB - (reply: 1)
268. Negative ascitic fluid - (reply: 1)
269. Human dendritic cell line - (reply: 5)
270. storage buffer for purified protein - (reply: 3)