software for overlapping IF images - (Jul/30/2008 )

Hi all,

I am using 3 different antibodies conjugated with different fluorophores to stain a tissue section. Does anyone knows what is the good software to combine 3 fluorophores pictures into one so that the 3 colors can be visualized in one picture. Thank you.

Regards,
William

-wllmch-

QUOTE (wllmch @ Jul 30 2008, 07:51 AM)

your microscope should be equipped with such a software; in Photoshop you can subsequently overlay images; the first two are to overlay with 50%, the third with 33%...; the disadvantage of this method with Photoshop is the loss of brightness...

-The Bearer-

i dont know, the microscope doesnt come with a software to combine pic. It is a pain.. was trying to use GIMP but looks complicated..

-wllmch-

I generally use Photoshop for merging mages. If you want to buy software then THIS should be a good one.

-Calvin*-

Hi, try to use ImageJ

You can download it on this web site (if you dont have it):

http://www.ansci.wisc.edu/equine/parrish/index.html

We routinely use it in our lab for merging the images.

-Veronika24-

QUOTE (Veronika24 @ Aug 8 2008, 08:48 AM)

Hi, try to use ImageJ

You can download it on this web site (if you dont have it):

http://www.ansci.wisc.edu/equine/parrish/index.html

We routinely use it in our lab for merging the images.

I totally agree!! Image J or the Mac equivalent of NIH image is really good for overlaying images as you described.

-labrat612-