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961. Immunostaining with chamber slides - (reply: 4)
962. Streptavidin-Alexa fluor 488 - it should be dissolved in PBS or PBS/BSA? - What concentration of it do you use, and how long is incubation? (reply: 2)
963. ELISA negativ control? - (reply: 2)
964. How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
965. Low ELISA titers - (reply: 1)
966. ELISAS: U-bottom plates vs. flat bottom? - (reply: 3)
967. cytokines in serum - (reply: 1)
968. Polyclonal immunoaffinity column - (reply: 2)
969. Dendritic cell novice - (reply: 3)
970. ELISA- triton in coating buffer - unconventional plate coating (reply: 5)
971. what's the defference of normal serum and whole serum? - (reply: 2)
972. Human IgG from protein G - (reply: 1)
973. T cell activation. - (reply: 1)
974. IF fixation - (reply: 3)
975. Background with chicken primary antibody - (reply: 2)
976. Cytokine/chemokine detection in culture supernatant - (reply: 6)
977. Ab purification from supernatant - dialysis of large volumes (2L) (reply: 3)
978. very higher background in ELISA which was going on very well before - (reply: 10)
979. Autofluorescence elimination from cell aggretates - Immunostaining troubleshoot: Autofluorescence (reply: 1)
980. Western blot background and specificity - How good is my antiserum? (reply: 1)
981. Anti Lamp-1 or Lamp-2 for COS cells? - (reply: 3)
982. ...the best antibodies? - (reply: 10)
983. antibody purification - (reply: 5)
984. Anti-His tag monoclonal antibody - (reply: 3)
985. help: macrophages transfection and immunofluorescence - (reply: 2)
986. Antibody adsorption protocol - Immunology (reply: 1)
987. Disadvantages of immunochemistry. - (reply: 1)
988. conservation of antibodies! - after purification! (reply: 5)
989. Apoptosis - caspase-3 activity (reply: 1)
990. Staining Brains (immunofluorescence) - Any tips to improve staining? (reply: 4)
962. Streptavidin-Alexa fluor 488 - it should be dissolved in PBS or PBS/BSA? - What concentration of it do you use, and how long is incubation? (reply: 2)
963. ELISA negativ control? - (reply: 2)
964. How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
965. Low ELISA titers - (reply: 1)
966. ELISAS: U-bottom plates vs. flat bottom? - (reply: 3)
967. cytokines in serum - (reply: 1)
968. Polyclonal immunoaffinity column - (reply: 2)
969. Dendritic cell novice - (reply: 3)
970. ELISA- triton in coating buffer - unconventional plate coating (reply: 5)
971. what's the defference of normal serum and whole serum? - (reply: 2)
972. Human IgG from protein G - (reply: 1)
973. T cell activation. - (reply: 1)
974. IF fixation - (reply: 3)
975. Background with chicken primary antibody - (reply: 2)
976. Cytokine/chemokine detection in culture supernatant - (reply: 6)
977. Ab purification from supernatant - dialysis of large volumes (2L) (reply: 3)
978. very higher background in ELISA which was going on very well before - (reply: 10)
979. Autofluorescence elimination from cell aggretates - Immunostaining troubleshoot: Autofluorescence (reply: 1)
980. Western blot background and specificity - How good is my antiserum? (reply: 1)
981. Anti Lamp-1 or Lamp-2 for COS cells? - (reply: 3)
982. ...the best antibodies? - (reply: 10)
983. antibody purification - (reply: 5)
984. Anti-His tag monoclonal antibody - (reply: 3)
985. help: macrophages transfection and immunofluorescence - (reply: 2)
986. Antibody adsorption protocol - Immunology (reply: 1)
987. Disadvantages of immunochemistry. - (reply: 1)
988. conservation of antibodies! - after purification! (reply: 5)
989. Apoptosis - caspase-3 activity (reply: 1)
990. Staining Brains (immunofluorescence) - Any tips to improve staining? (reply: 4)