plate bound vs. soluble anti-CD3 - (Jul/12/2006 )

Can anyone advise on use of plate bound versus souble anti-CD3 for T cell activation?

A few specific questions...

Which activates memory T cells?
Which activates naive T cells?
Does using anti-CD28 also affect these differential effects?
Do the different methods affect APCs (in spleen culture) in any way?

Any help appreciated...

D.

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There is a paper that describes in great details the use of dynabeads coated with appropriate amt of anti CD3 and Anti-CD28. I will post the reference tomorrow when I get to the lab. these beads will actually act as an artifical APC. I plan on making them and they can be stored at +4 for 4 months. in my case I intend to stimulate PBMNCS for 6 hrs with a viral antigen and the CD3 CD28 beads. I want to monitor both CD4 and CD8 activities. to measure activation I would do a flow and stain for CD62L and CD69

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Heres the ref: Riddell and Greenberg, J Immnol Methods, 1990 , Vol 128, pp189-201

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