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241. Protein shows KD but RNA level is higher - (reply: 4)
242. miRNA spike-in control - (reply: 6)
243. shRNAs work in vitro, not in vivo - (reply: 12)
244. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
245. miRNA 3ŽUTR target expression vectors - (reply: 6)
246. shRNA - (reply: 4)
247. nucleic acid extraction from silenced cells - (reply: 6)
248. Is there any pre-miRNA located in exon? - (reply: 1)
249. Differences between lentiviral packaging kits - (reply: 1)
250. siRNA overhangs - (reply: 2)
251. Can miRNAs regulate miRNA expression - (reply: 3)
252. Problem in shRNA Annelaing ( Help Required ) - (reply: 2)
253. siRNA in the Fridge - (reply: 2)
254. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
255. miRNA directed upregulation?! - (reply: 3)
256. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
257. Transfection - (reply: 1)
258. Double transfection of siRNA - (reply: 2)
259. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
260. how to expression mirna in a specific tissue ？ - (reply: 1)
261. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
262. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
263. the necessity of microRNA mutation design - (reply: 1)
264. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
265. transfection efficiency - (reply: 1)
266. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)
267. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
268. Dicer knockdown - (reply: 6)
269. miRNA clusters - miR-19 (reply: 1)
270. short 3'UTR - microRNA target (reply: 5)
242. miRNA spike-in control - (reply: 6)
243. shRNAs work in vitro, not in vivo - (reply: 12)
244. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
245. miRNA 3ŽUTR target expression vectors - (reply: 6)
246. shRNA - (reply: 4)
247. nucleic acid extraction from silenced cells - (reply: 6)
248. Is there any pre-miRNA located in exon? - (reply: 1)
249. Differences between lentiviral packaging kits - (reply: 1)
250. siRNA overhangs - (reply: 2)
251. Can miRNAs regulate miRNA expression - (reply: 3)
252. Problem in shRNA Annelaing ( Help Required ) - (reply: 2)
253. siRNA in the Fridge - (reply: 2)
254. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
255. miRNA directed upregulation?! - (reply: 3)
256. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
257. Transfection - (reply: 1)
258. Double transfection of siRNA - (reply: 2)
259. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
260. how to expression mirna in a specific tissue ？ - (reply: 1)
261. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
262. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
263. the necessity of microRNA mutation design - (reply: 1)
264. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
265. transfection efficiency - (reply: 1)
266. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)
267. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
268. Dicer knockdown - (reply: 6)
269. miRNA clusters - miR-19 (reply: 1)
270. short 3'UTR - microRNA target (reply: 5)