how to expression mirna in a specific tissue ？ - (May/21/2010 )
I want to express a mirna in the liver of zebrafish ？
How to build this kind of vector?
thank you
-luc-
You will need to clone the precursor miRNA sequence (the pre-miRNA sequence plus 100-200 bp flanking sequences on both sides) into a expression vector and the transcription of the miRNA in the vector should be driven by a tissue (liver) specific promoter.
-pcrman-